running a gel with primers - (Dec/08/2006 )

Is it possible to run a gel using plasmid DNA that already has primers in it and is restriction digested? Or is this usually done PCR instead of RE digestion? Thanks.

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I'm not sure what your goal is...I run my restriction digest mixture on a gel to gel extract all the time, but if I'm not doing a digest, I will PCR purify before running my gel. Does that help?

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well it's possible to run a digested PCR product with primers, if one of the expected bands are not around 100.
If one is 200, a 3% agarose gel can efficiently discriminate between it and primers.

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