Gel extraxction question - (Jun/27/2005 )

I made lots of gel extraction using QIAquik kit, and i never had problems.

but

last time i found something strange in my extract... it seems gel!!!

how is it possibile?

-Dees-

I have used these same kits and found them to be quite reliable. Perhaps you got a bum column with a crack or hole in the frit allowing the agarose to flow thru?

-pBluescript-

nononono

i followed the protocol, but this time something didn't work....

i had 2 tubes, and in both i found gel

(2 different spin columns, sterile conditions etc etc)

-Dees-

QUOTE

in both i found gel

.

By gel, do you mean actual gel, or white powdery stuff that looks like agarose?

-pBluescript-

I mean white powdery stuff that looks like agarose...

-Dees-

I am sticking to my story of column material that is flowing thru an imperfection in the column frit.

Spin the eluate 5 min 14k xg and take the supernate. Discard the pellet of white stuff. Check your plasmid on a gel.. if all looks good then go.

-pBluescript-

imperfection? in 2 different colums?

I worked out repeating the extraction following the protocol again.

-Dees-