No peak for gel filtration for soluble protein - Need help (Mar/07/2005 )

I purified a MBP-tagged protein, which is quited soluble. On SDS-PAGE, the MW is about 65 KDa and it's a very thick band. However, when I run the protein elution on gel filtration, both 75 and 200 column, there is no any peak for protein. The running buffer I used is the same as elution buffer for purification.
I really don't know what happened? Did my protein degraded or it's stucked on the column?

Thanks for your help! I am really frustrated about this.

Sophia

Are you confident in the loading procedure of gel filtration?
in my lab, some labmate load the protein wrongly and result in loss of all protein