Gel purification: SpinX vs commercial kits - Gel purification (Oct/24/2007 )

Does anybody have experience using the SpinX columns and the commercial kits like QiaEx for purification of DNA bands from agarose gel? Would appreciate if yo u can share your experience. I am looking for a method that gives good recovery (product size 10 kb), and with as little fuss as possible.

thanks!

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If you have the funds, the Qiagen kit is extremely easy to use (I like the QiaQuick gel extraction kit). Provided you run a large prep gel, and cut the bands out VERY closely under UV light, this kit will give you a reasonably high yield with very little fuss.

Best of luck!

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I vote for the Qiagen kit. The recovery is good i feel only fuss is drying the beads.
All the best

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Freeze the DNA band in agarose at -80 degree for more than one hour, thaw on ice, cntrifuge and collect supenatant. This DNA is good for ligation followed by transformation. You could futher purify using phenol-chloroform extraction followed by ethanol precipitation. You should roughly get recovery of 50% or more before purification. Typical spin cloumn from sigma would give 10-30% recovery.

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