Best Brand of Agarose? - (Sep/12/2005 )

What type of agarose does everyone think is the best? We are having troubles with ligation efficiency in the lab and my PI thinks that it might be some carry-over inhibitor form the agarose after we gel isolate with Qiagen Spin Columns?

It is easier to find a better purification kit rather than a better agarose gel.

I agree, it is probably something else affecting the ligation reaction; eg ATP gone bad. If your DNA is purified, it should be clean no matter where you get your agarose from.

I suspected from long time that qiagen gel extraction kit is not adviced for the purif for further ligation, so I ommited such step as far as possible. Make just a cut, purify it from the enzymes, and ligate all that you can together, later, in the screening, you will choose, what you need.