Native PAGE gel - (Jul/01/2008 )

Hello,

I am hoping to get some help regarding a native gel. Our protein runs about 600 kD, and running them on a normal Tris-Glycine system has proved to be challenging as the protein takes a while to run. On Tris-Acetate gels from Invitrogen, it takes about 2 hours to run. Can someone provide a possible recipe to make a native gel that has the ability to run large proteins in an efficient manner?

Thanks,
dave

you can find native gel formulations within this forum (i don't remember exactly where but you can search). you just have to decide what acrylamide concentration you should use for your protein (5% or a 4-10% gradient should be okay).