Gel extraction - just wondering!!!! (May/08/2006 )

Hi there all,

Just wondering, im using the Qiagen Gel Extraction Kit, i have extracted the desired band since i do face unspesific binding. Is it ok to keep the extracted gel in 4C overnight and carry on the process the next day????

Appericiate your views,

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Hi,
I have tried this out when i got busy with some other work. The gel slice can be very well stored at 4C ( i stored till 24hrs) and it worked fine for me. There was no DNA damage or loss due to this.


Vineet

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Did the same thing too.. kept my gel overnight at 4 degress.. able to use still...

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Did the same thing today, and there was no result beyond expectation.

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Thanks loads, cause i was really busy today. I extracted the gel at bout 8pm. I'll do the extraction tomorrow morning. I think about 12 hrs different. Hope the result will b the same.

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I normally store the gel at -20C for longer period of time, during weekend or for a week or so. There is no loss of DNA.

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i agree with scolix. there is no problem storing the gel slice at -20°C. i have done it several times before and there was no lost of dna observed
flausch

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Theoretically the only problem would be the presence of exonucleases "eating" the overhangs from the cut DNA. So I would put it in -20

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I kept my gel slice o/n in -20 and used next day w/o any problem!

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