RNA Storage - (Aug/10/2007 )
How long will RNA keep at -80?
well, if your frezer does not have breaks and if the freezer is properly used (never allowed to get more than -71°C, your RNA would probably be ok for many years.
It can be stored for years if stored at -80C without major temp. fluctuations.
some days before we had a terrible earthquack and there was no electricity for all day long.
at that time we run our -80 fridger with a small generator..........the temperature of the fridger was downed to -70c for some hours. is that a problem for RNA storage??
at that time we run our -80 fridger with a small generator..........the temperature of the fridger was downed to -70c for some hours. is that a problem for RNA storage??
I have a similar question - How is RNA usually stored? In TE? With DEPC or not? Etc?
we used to store RNA in DEPC water.
-70°C should be fine as it's considered limit of biological activity (see cryopreservation related topics)
So i guess the RNA is still intact. If you have an aliquot you may test it, but if not's the case, don't worry.
I used to store RNA in DEPC water, but now, i prefer in purchased ultrapure water and aliquoted
Actually, we keep RNA samples at -80 C for years. We also store in ultrapure (nuclease-free) water. It is better to alliquot your samples and thus you can use alliquots for some measurements or treatments without harming your stock store.
I've done quantification on RNA samples stored in DEPC water at -80 that were over 2 years old with little degradation. However, it is preferable to convert samples to cDNA for long-term storage.