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Promoter studies - (Jan/03/2008 )

Hi Guys

Its me again,
I have cloned the promoter of my gene. is there any way to know if someone has cloned and published it before . Is it possible to publish the f promoter structure like TSS , or important transcription factors or where TATA box is ?? etc.

thanks in advance.

regards
Vani

-vani.khare-

What organism are you working on, human, mouse? Even you have fully characterize the promoter experimentally chances are low to publish in good journals. Characterizing a promoter nowadays is not something that pays off well. You have to do experiments such as footprinting, gel shifting, primer extension, ChIP assay, many of them require radioactive work.

-pcrman-

QUOTE (pcrman @ Jan 3 2008, 08:54 PM)
What organism are you working on, human, mouse? Even you have fully characterize the promoter experimentally chances are low to publish in good journals. Characterizing a promoter nowadays is not something that pays off well. You have to do experiments such as footprinting, gel shifting, primer extension, ChIP assay, many of them require radioactive work.


Thanks PCRman for responding to my quest.......Actually I m done with EMSA and m in the middle of transiant transfection using this promoter . but I just wanted to know if structure of promoter(bioinformatics) can also go in a paper together with other results. And how to know that this promoter is not analyzed before ??
I mean is there any site where they submit promoter sequence or some database?? my promoter is from Zebrafish.

cheers
Vani

-vani.khare-

the zebrafish genome has been published. Go to UCSC genome browser or ENSEMBL to look it up.
The sequence of everything is published in database.

-mikew-