Protocol Online logo
Top : Forum Archives: : Molecular Biology

PGK-NEO whole sequence - (Feb/27/2008 )

Is there anyone who has the whole sequence of PGK-NEO gene fragment? I did the sequencing of one reaction and get about 1kb sequence. Can any body offer the whole? I really appreciate your help.

-wuyu-

here is pgk PURO

1 GCACACGTGC AGGCACCGGG CTTGCGGGTC ATGCACCAGG TGCGCGGTCC
51 TTCGGGCACC TCGACGTCGG CGGTGACGGT GAAGCCGAGC CGCTCGTAGA
101 AGGGGAGGTT GCGGGGCGCG GAGGTCTCCA GGAAGGCGGG CACCCCGGCG
151 CGCTCGGCCG CCTCCACTCC GGGGAGCACG ACGGCGCTGC CCAGACCCTT
201 GCCCTGGTGG TCGGGCGAGA CGCCGACGGT GGCCAGGAAC CACGCGGGCT
251 CCTTGGGCCG GTGCGGCGCC AGGAGGCCTT CCATCTGTTG CTGCGCGGCC
301 AGCCGGGAAC CGCTCAACTC GGCCATGCGC GGGCCGATCT CGGCGAACAC
351 CGCCCCCGCT TCGACGCTCT CCGGCGTGGT CCAGACCGCC ACCGCGGCGC
401 CGTCGTCCGC GACCCACACC TTGCCGATGT CGAGCCCGAC GCGCGTGAGG
451 AAGAGTTCTT GCAGCTCGGT GACCCGCTCG ATGTGGCGGT CCGGATCGAC
501 GGTGTGGCGC GTGGCGGGGT AGTCGGCGAA CGCGGCGGCG AGGGTGCGTA
551 CGGCCCTGGG GACGTCGTCG CGGGTGGCGA GGCGCACCGT GGGCTTGTAC
601 TCGGTCATGG TAAGCTAGCT TGGGCTGCAG GTCGAAAGGC CCGGAGATGA
651 GGAAGAGGAG AACAGCGCGG CAGACGTGCG CTTTTGAAGC GTGCAGAATG
701 CCGGGCCTCC GGAGGACCTT CGGGCGCCCG CCCCGCCCCT GAGCCCGCCC
751 CTGAGCCCGC CCCCGGACCC ACCCCTTCCC AGCCTCTGAG CCCAGAAAGC
801 GAAGGAGCAA AGCTGCTATT GGCCGCTGCC CCAAAGGCCT ACCCGCTTCC
851 ATTGCTCAGC GGTGCTGTCC ATCTGCACGA GACTAGTGAG ACGTGCTACT
901 TCCATTTGTC ACGTCCTGCA CGACGCGAGC TGCGGGGCGG GGGGGAACTT
951 CCTGACTAGG GGAGGAGTAG AAGGTGGCGC GAAGGGGCCA CCAAAGAACG
1001 GAGCCGGTTG GCGCCTACCG GCAGCTGTGC

just replace puro sequence by neo one. I can't access my accurate files properly for instance sorry...

-fred_33-

Thank you very much!

-wuyu-