ligation question - ligation (Jan/13/2005 )

can anybody tell me, is it useful to run the ligation mixin the gel?really confused since somebody suggested to do this step. Also, the Insert : Vector (mol)=? be better, 1:1; 1:3; 3:1????????or depend on your stuff?thanks.

Do you mean to run a gel using the ligation reaction? It won't hurt if you do so and may give you some idea whether your ligation is successful or not.

I do not think you can make sure of your ligation from the gel .I did like this,though I can see three different bands(insert,digested vector,and after that was another band,which seems to be the ligation),but I got no right colony !
But it will not give any side effect by doing that ,and the ligation mixture will be useless after transformation,so ,you can have a try .