please help me to be sure from design primer have it - (Aug/12/2008 )

please help me
if any one can help me ,how to be sure from one primer someone design it to me iis correct ,althought i have some program to design primer , can any software help me to be sure from the sequence i have it

-mazan-

check the sequence with blast nucleotide (pubmed) it will tell you all the sequence that are similar to it

-merlav-

Hi Vector NTI is the best choice for you , insert complete sequence to this softwware and find place where it hybridize, you can create primer in this software and other things. I have designed multiple primers and all work
good luck

-baxapoptoaia-

Check out free tools for PCR Primer on the internet

Primer3
http://fokker.wi.mit.edu/primer3/input.htm

Oligo Calc: Oligonucleotide Properties Calculator
http://www.basic.northwestern.edu/biotools/oligocalc.html

The first one good at picking primers. Second one helpful for analysing the Primers for Tm, Dimers or hair pins, etc

All the best

Regards
Avinash

http://aviprem.gq.nu

-aviprem-