BclI working efficiency - enzyme cutting (Feb/24/2005 )

Can BclI cleave the sequence that close to the end of DNA fragments.

---

hi
as said by NEB, it seems that this RE require 6 bases pairs on either side of their recognition site to cleave efficiently.
I hope it's compatible with your experiment.

---

thanks for you reply, but in my experiment, i only have 2 base left on the right side, has anyone had experience about the enzyme in this situation.

---

then you will experience reduced digestion efficiency. Try redesigning your scheme to accomodate these extra bases.

---

"reduced" can mean "none" here, if that's not been tested by someone, noone can predict what activity the enzyme will show. so it's best to follow george@CASE' s advice and try to use another stategy or primer!

mike

---

thanks for all your answer, I will change me experiment strategy.

---