Extracting DNA from serum - (Jan/20/2006 )

Hi i need some help:

i have been given the task of finding a way of extracting DNA from sera without using a qiagen kit. Does anyone know of any protocols or papers relating to this?

Thanks

-bsuac6-

do you mean without a QIAgen kit, or without any commercial kit (ie using homebrew buffers)?

-John Buckels-

QUOTE (John Buckels @ Jan 20 2006, 05:35 PM)

do you mean without a QIAgen kit, or without any commercial kit (ie using homebrew buffers)?

Without any commercial kit.

thanks

-bsuac6-

Hi bsuac6,

Maybe it's not the safest protocol but I've always used phenol-chloroform for DNA purification from Serum and Plasma and always obtained better yields than with any other commercial kit (I'm not very fond of them, I have to say... ). Maybe purity is not as good but I've detected very small quantities of DNA using PCR after DNA purification.

It has always worked for me...

Esther

-esola78-

I agree with Esther. Phenol/chloroform is and always has been my favourite method for isolating DNA.

-John Buckels-

Thanks guys, i tend to use phenol/chloroform with whole blood, so i guess i might as well use it for sera.

Thanks again.

-bsuac6-

QUOTE (esola78 @ Jan 23 2006, 11:16 AM)

). Maybe purity is not as good but I've detected very small quantities of DNA using PCR after DNA purification.

It has always worked for me...

Esther

Hi,

I have:

od 260 od 270 od 280

0,066 0,103 0,044

What's the meant of these data?
I know that there is protein contamination, but I don't know the meant of this od 270....
I used 2 cycles of PCI extraction and 1 cycle of microcon 100......

Thanks!

-flavio-