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Antibody Related Discussions
  1. Western Blot - Any one ever use serum as a primary antibody? (reply: 2)
  2. Raising monoclonal antibodies - Monoclonal antibodies raised against similar sequence Antigens in mice (reply: 2)
  3. Dilution Buffer for Elisa - Dilution buffer for titre check of polyclonal serum or for monoclonal (reply: 2)
  4. antibody titer - (reply: 1)
  5. About DNMT1 antibody - (reply: 1)
  6. which part of an antigen should monoclonal antibodies bind to? - how to choose appropiate epitopes? (reply: 1)
  7. Actin Antibody short-lived? - (reply: 2)
  8. Co-immunoprecipitation - Source of cheap irrelevant antibody (reply: 2)
  9. What do u think about my protocol for EMSA with antibodies? - EMSA experts out there??? (reply: 1)
  10. Antibody out of stock: ab8580 - (reply: 4)
  11. How long can I leave the coating antibody on? - (reply: 5)
  12. Secondary antibody - (reply: 2)
  13. So what is fluorescence SUPPOSED to look like? - fluorescent secondary antibody scans as SOLID BLACK image (reply: 1)
  14. antibody against His-tagged protein - (reply: 3)
  15. Antibodies, ascites and antisera - (reply: 1)
  16. Visualizing CpG island in IF - Are there antibodies available? (reply: 1)
  17. western blot, I have a signal without primary antibody - (reply: 6)
  18. mAB vs pAB - (reply: 4)
  19. Combine 2 different primary antibodies produced by the same animal - Is it possible to succeed the impossible? (reply: 7)
  20. Non-specific bands with secondary Antibody on Western Blotting - (reply: 1)
  21. Monoclonal Ab Western and ELISA issue - (reply: 2)
  22. Blocking antibody - (reply: 1)
  23. monoclonal antibody (mAb), mouse, IgG2a - Urgent (reply: 3)
  24. HSV1-Tk (mono/ployclonal) antibody. - (reply: 1)
  25. Finding an antibody for a weird species ! - What matters in protein blast? identity or positive? (reply: 1)
  26. anyone knows good HA antibody for zebrafish whole mount stainging - (reply: 4)
  27. ELISA for antibody titre using Insoluble recombinant protein - Newbie question (reply: 1)
  28. Antigen coated beads - For making antibodies (reply: 1)
  29. Purificiation of Salmonella antibodies - (reply: 3)
  30. antibody to use for methylase chip - DNMT/MBD/MeCP2? (reply: 3)
  31. Looking for nucleotide sequence of rearranged antibody gene locus. - (reply: 1)
  32. Immobilisation of Antibodies to Au ( Gold) surface - (reply: 2)
  33. mouse strain for monoclonal Ab - (reply: 2)
  34. antibodies working in indirect ELISA but not in inhibition ELISA - (reply: 11)
  35. Dose someone have the protocol of antibody production? - (reply: 1)
  36. Bacterial expression antibody recognition problem - antibodies won't recognize protein (reply: 2)
  37. Obtaining Conjugate Antibody for Sandwich ELISA - How do I obtain/create the appropriate Alkaline-phosphatase Antibody (reply: 7)
  38. Why do I get non-specific bands? - with my secondary antibody.... (reply: 5)
  39. Why have I bullet shaped bands on my Antibody SDS Page - (reply: 1)
  40. How to release antibodies from beads after IP? - in order to run ELISA after IP, not Western Blot. (reply: 7)
  41. where to get custom antibodies? - (reply: 1)
  42. Negative control for primary antibody in immunohistochemistry - What is it? (reply: 9)
  43. what kind of secondary antibody do i need? - (reply: 1)
  44. Coating with monoclonal/polyclonal - (reply: 6)
  45. how to prepare blocking antibody - (reply: 1)
  46. Digestion of antibody using pepsin - no F(ab)2 was found (reply: 1)
  47. Antibody reuse for Western blot - (reply: 7)
  48. Control for primary antibody in Immunohistochemistry - What is it? (reply: 4)
  49. Phosphatase treatment as a control for Phospho antibodies - (reply: 1)
  50. recombinant human antibody production problem - (reply: 2)
  51. MHC II molecules - antigen bound state - purchasable conformation specific antibodies (reply: 1)
  52. How to reduce amount of antibodies used in WB - (reply: 4)
  53. Can we re-use antibody solutions just like Western Blot? - to save antibody (reply: 3)
  54. Monoclonal vs Polyclonal Abs - (reply: 1)
  55. probing one protein with multiple antibodies - (reply: 2)
  56. Resuspending lyophylized antibodies - (reply: 1)
  57. age of antibodies - (reply: 1)
  58. antibody storage - how to store? (reply: 7)
  59. using two antibodies at the same time in one blot - (reply: 4)
  60. Custom polyclonal peptide antibody services? - (reply: 4)
  61. Best Antibody for CHiP (H3K4 and H3K27) - Looking for the best antibody for histone CHiP (reply: 1)
  62. gel and monoclonal antibody issues - (reply: 3)
  63. How to detect if you've got antibody in your tube? - I want to know if my sample is really a monoclonal antibody! (reply: 11)
  64. How to reuse antibody in Western blotting - Share your experience (reply: 8)
  65. IP with Monoclonal Antibodies - (reply: 1)
  66. Is my antibody specific? - (reply: 3)
  67. Is my antibody specific? - (reply: 1)
  68. Can we reuse the primary antibody in immunohistochemistry? - (reply: 3)
  69. Antibodies for flow cytometry - MAP2, NeuN, GFAP (reply: 4)
  70. Testing the primary antibody - Is the secondary antibody recognizing the primary (reply: 1)
  71. monoclonal antibody or polyclonal antibody? - which is better for ChIP? (reply: 1)
  72. Polyclonal antibody service - Who can you recommend? (reply: 2)
  73. what does "(H + L)" mean in the secondary antibody? - (reply: 3)
  74. Concentrating antibodies - (reply: 3)
  75. KLH-based immunization protocols: suitable for hybridoma production purposes? - (reply: 4)
  76. What is needed for a hybridoma growth culture - (reply: 1)
  77. How dilute an antibody 1:150000 - (reply: 2)
  78. Santa Cruz antibody - (reply: 14)
  79. WB antibody to be used in IHC - (reply: 7)
  80. Help: New to biochemistry and need help with antibody? - (reply: 11)
  81. mouse and rabbit antibodies in western blot - (reply: 2)
  82. affinity determination of an antibody - (reply: 5)
  83. How to dilute an antibody? - (reply: 2)
  84. Hybridoma cultures - floaters? - (reply: 4)
  85. how to label an antibody - (reply: 3)
  86. polyclonal vs monoclonal - (reply: 2)
  87. Help choosing an anti-human antibody - (reply: 2)
  88. Can I incubate membrane with 2 antibodies at the same time ? - (reply: 5)
  89. Specifity of antibodies - (reply: 2)
  90. Western Blot Background - Having high backgrounds on western blotting due to antibody issues. (reply: 2)
  91. which conjugate for the secondary antibody in immunohistochemistry - (reply: 1)
  92. crosslinking antibodies before IP - (reply: 2)
  93. ELISA Problem since change of antibody supplier? - (reply: 1)
  94. mouse anti-goat antibodies - (reply: 1)
  95. antibody gold labeling - (reply: 3)
  96. Monoclonal antibody affinity (Kd constant) determination using ELISA - (reply: 2)
  97. Antigen Anti body and Protein - (reply: 3)
  98. Can an antibody just stop working? - (reply: 5)
  99. Decrease in serum antibody titers - (reply: 3)
  100. HA antibody - (reply: 1)
  101. Antibody help please - (reply: 3)
  102. Diluting/mixing of antibodies - exact method? - (reply: 1)
  103. Opinion about fluorescent antibodies - (reply: 6)
  104. Blocking for phospsho antibodies - (reply: 4)
  105. secondary antibody labeling with NHS ester functionalized help? - (reply: 1)
  106. Apoptosis detection with Phosphatidylserine antibody - (reply: 4)
  107. Qiagen penta-HIS antibody not reusable - looses activity after freeze-thaw-cycles (reply: 2)
  108. Frozen Antibody Lifespan - (reply: 2)
  109. polyclonal antibodies using inclusion bodies - how to prepare sample (reply: 1)
  110. anti-gfp antibodies - (reply: 4)
  111. labelling primary antibody with fluorescent tag - (reply: 1)
  112. Hybridoma culture not secreting mouse antibody - (reply: 6)
  113. How much of antibody to add? - (reply: 3)
  114. mouse anti-mouse antibody ? - (reply: 5)
  115. ELISA - coating antibody (reply: 3)
  116. Which p-Serine antibody is suitable for IP and WB - (reply: 1)
  117. looking for TNF alpha antibody for human tissue IHC - (reply: 1)
  118. "recombinanant antibody" - (reply: 3)
  119. production of monoclonal antibody when the host protein is having sequence simil - (reply: 3)
  120. How about the antibody quality of these two companies? - antibody quality (reply: 5)
  121. secondary antibody? - (reply: 4)
  122. HELP! confusing antibody - (reply: 14)
  123. preferred method for purifying antibodies - (reply: 2)
  124. Denaturation of antibodies in high salt - (reply: 3)
  125. antibodies testing - (reply: 4)
  126. problem with anti-acetyl H3 antibody - (reply: 11)
  127. how to select the proper antibody - (reply: 5)
  128. IP problems - The antibody doesn't bind to the beads (reply: 5)
  129. fluorescent secondary antibodies --> reccomendations? - (reply: 6)
  130. polyclonal antibodies go bad - when stored at -80???? (reply: 5)
  131. antibody incubation on western blots - difference between 4°C and RT (reply: 10)
  132. shape-shifting antibodies? - (reply: 11)
  133. My antibody does not work for IP, but works well fpr western? - antibofy not working for IP (reply: 12)
  134. Making antibodies - Anybody has designed a custom antibody? (reply: 4)
  135. What are the differences between monocloan and polyclonal antibody? - help! (reply: 1)
  136. stripping antibodies in immunochemistry? - (reply: 6)
  137. Affinity Purified Antibody - (reply: 1)
  138. antibody against yeast protein - (reply: 1)
  139. reusing PVDF membrane for different antibodies without stripping - (reply: 6)
  140. Antibodies for immunohistochemistry and cell ELISA - Are they the same? (reply: 2)
  141. purification of HRP antibody conjugate - (reply: 10)
  142. antibody classification - (reply: 1)
  143. Freezed antibodies - (reply: 2)
  144. storage of ammonium sulphate purified antibodies - (reply: 1)
  145. native or denatured protein? - for antibodies (reply: 5)
  146. Antibodies for different epitope giving bands of different sizes in the same sam - (reply: 1)
  147. Antibodies to stain vesicles/vacuoles in my cells? - (reply: 4)
  148. Do antibody solutions settle? - (reply: 2)
  149. antibody labelling for immunochromatographic assay - (reply: 4)
  150. creating a neg. charge on an Antibodies' Fc region - (reply: 2)
  151. Looking for primary antibody to BiP (grp78) for flow cytometry - (reply: 2)
  152. Why should some antibodies not be aliquoted? - (reply: 12)
  153. Primary-Secondary Antibody Question - (reply: 5)
  154. RNA polymerase II antibody recommendation? - (reply: 2)
  155. protein estimation of monoclonal antibody - inconsistent values (reply: 2)
  156. Mab purification - no mouse IgG band seen (reply: 3)
  157. Confirming IP with WB - Can I use the same antibody for both IP and WB? (reply: 5)
  158. Monoclonal screening of stable cells - How to maintain it to confluent stage? (reply: 4)
  159. Antibody not binding to protein A/G - Immunoprecipitation (reply: 3)
  160. macrophages collection for hybridoma - (reply: 1)
  161. Is it OK to use monoclonal antibody against its host protein for westen - (reply: 4)
  162. Couple peptide to beads? for antibody purification - (reply: 1)
  163. Did my Ab provider do me wrong? - Judging the purity of my antibody (reply: 3)
  164. Looking for an antibody/epitope type of search... - (reply: 1)
  165. Could protein modification block an antibody epitope? - (reply: 4)
  166. polyclonal antibody production - is it necessay to remove fusion tag? (reply: 2)
  167. Antibody for EMSA - (reply: 2)
  168. pSTAT5 antibody - (reply: 5)
  169. Is my secondary antibody the cause for non-specific signal? - (reply: 3)
  170. Ki67 Antibodies - (reply: 6)
  171. Anti-hunman CD monoclonal antibody - (reply: 1)
  172. Antibody detecting albumin - (reply: 1)
  173. anti-flag antibodies - (reply: 6)
  174. lyophilization of monoclonal antibodies... How? - (reply: 5)
  175. Antibody storage - (reply: 4)
  176. Affinity purification of antibody - (reply: 2)
  177. Antibodies and triton X - (reply: 4)
  178. conformation specific monoclonal antibody - how to confirm? (reply: 4)
  179. Problem with Monoclonal antibody production - why cells die after adding HAT?????? (reply: 6)
  180. antibody purification - how to get rid of bovine IgG? (reply: 3)
  181. Stability of primary antibodies at room temperature - (reply: 4)
  182. Antibodies for ChiP - Of mice and men (reply: 1)
  183. Oligodendrocyte's and tubulin antibody in Western Blot - (reply: 1)
  184. polyclonal antibody production protocol - looking for protocols (reply: 2)
  185. Good GFP antibody from SANTA CRUZ for Western Blotting? - (reply: 7)
  186. monoclonal antibody production - (reply: 6)
  187. Catalase Antibody - (reply: 2)
  188. Human TERT Antibody - (reply: 2)
  189. Need clean HAtag antibody for mouse tissue lysates - (reply: 1)
  190. humanizing an antibody? - (reply: 5)
  191. Catalase Antibody - (reply: 1)
  192. Polyclonal antibodies in immunocytochemistry not working - (reply: 4)
  193. FLAG antibodies for mouse samples - Immunofluorescence/Flow cytometry (reply: 1)
  194. Multiple antibodies in Western - (reply: 5)
  195. ELISA/ BSA in capture antibody storage buffer - (reply: 3)
  196. Are there antibodies to differentiate epithelial from fibroblasts cells? - (reply: 5)
  197. need antibody in TX - (reply: 1)
  198. Rainbow MW marker from Amersham - detection with a mouse secondary antibody? (reply: 1)
  199. using IgM antibodies in IP - (reply: 3)
  200. EMSA witj antibodies against TFII factors - (reply: 3)
  201. Monoclonal antibodies production older mice - (reply: 1)
  202. c-Myc antibody for Western - (reply: 1)
  203. Working antibody for human NFkB (p65) ChIP assay ? - (reply: 2)
  204. Antibody concepts - (reply: 20)
  205. Stable cell line, polyclonal and monoclonal. - Which is better? (reply: 4)
  206. antibodies life - 4oC, -20oC or -80oC (reply: 26)
  207. How to calculate antibody concentration from OD280 readings? - (reply: 2)
  208. Antibody produced in same species as target tissue - Will it cross react? (reply: 2)
  209. Does this antibody work? - (reply: 3)
  210. Western Blotting using Anti α Tubulin antibody: Bands too high! - (reply: 3)
  211. Antibody stability and additives - (reply: 4)
  212. ChIP IgG has the same signal than the antibody tested - (reply: 9)
  213. Best Chromatin IP antibody?(tag) - (reply: 3)
  214. NeuN and Ox-42 antibodies - (reply: 4)
  215. how to check protein-primary antibody binding - Is there any method or technique to check or proof read the primary Ab (reply: 7)
  216. antibody against denatured protein? - basic questions (reply: 2)
  217. Disaster! - Wrong temperature for antibody (reply: 9)
  218. Immunofluorescence using polyclonal antibody - (reply: 5)
  219. Weak and variable signal blotting for MDR1 using C219 monoclonal Ab - (reply: 2)
  220. monoclonal ab recognize one or more epitopes? - (reply: 4)
  221. Co-immunoprecipitation - antigen elution without the antibody - (reply: 4)
  222. Smearing antibody and DTT - how to interpret the gel? - Aggregated antibody (reply: 1)
  223. secondary antibody - (reply: 2)
  224. Antibody Mismatch Tolerance - (reply: 2)
  225. Storage of diluted antibodies - (reply: 6)
  226. Problem in IP (too high background) - mAb shows a lot of backgroud+light chain. (reply: 3)
  227. Antibody crossreactivity - (reply: 1)
  228. estimation of concentration of Ab in hybridoma culture supernatant? - (reply: 4)
  229. Antibody treatment - (reply: 2)
  230. can u guess the molecular weight of a raised antibody? - (reply: 3)
  231. Antibody storage in 5% milk - (reply: 1)
  232. dilution buffer for ap labeled 2nd antibody - (reply: 1)
  233. antibody cocktails - (reply: 4)
  234. does 2nd antibody concentration matters? - (reply: 3)
  235. Phage Display: Not getting antibodies after 3 rounds of panning - (reply: 6)
  236. same proteins, same antibodies, different results - (reply: 1)
  237. antibodies from hybridoma - (reply: 6)
  238. Shipping antibodies - (reply: 4)
  239. Freeze-thaw effects on antibodies and samples - (reply: 1)
  240. Western blottting - Santacruz antibodies - (reply: 10)
  241. Double staining antibody plus Annexin! - Help (reply: 2)
  242. Can anyone recommend the best FLAG and c-myc antibodies for co-IP? - (reply: 4)
  243. A great case problem on antibody assay - (reply: 2)
  244. Anybody has a good phosphorylated IKKa antibody? - (reply: 3)
  245. Antibody Dilution - (reply: 10)
  246. ASK1 antibody - (reply: 2)
  247. What is the sensitivity of dot blot compared to ELISA, and what antibody concent - (reply: 1)
  248. Antibody purification protocol --any suggestions? - (reply: 12)
  249. hrp labeling of antibodies - (reply: 3)
  250. oligopeptide beased antibody for IP - (reply: 2)
  251. NGF deprivation: why anti-NGF antibody? - (reply: 4)
  252. vortexing of antibodies upon dilution in 3% skim milk - (reply: 3)
  253. My antibody is forming a precipitate! - Why? (reply: 7)
  254. antibody concentration - antibody precipitation using ammonium sulfate (reply: 2)
  255. question on NF-kB antibody - (reply: 2)
  256. Concentration of antibody - (reply: 5)
  257. antibody - (reply: 4)
  258. specific ser/Thr phosphorylation detection by in house antibody - (reply: 1)
  259. Selection of Antibodies for CHip - (reply: 7)
  260. humanized antibody - how to design primers for CDRs - humanized antibody (reply: 1)
  261. help mAb - monocloanal antibodies (reply: 2)
  262. Antibody question - (reply: 5)
  263. Removing LPS from mAb stocks - (reply: 1)
  264. Polyclonal antibody purification? - how? (reply: 9)
  265. Multiple Bands in Westren Blot? - My antibodies are polyclonal...is dat d reason im getting multiple bands? (reply: 2)
  266. How can I elute antigen from proteinG+antibody+antigen complexes? - (reply: 3)
  267. antibody purification - how to purify from serum free medium (reply: 1)
  268. questions about secondary antibody - (reply: 2)
  269. Antibody from short peptides - Time saving, but do they work for both West and ELISAs (reply: 4)
  270. Rabbit IgG Isotype Control for Co-ip - Need available antibodies (reply: 1)
  271. Basic questions.. - antibody titer (reply: 5)
  272. Problem with antibodies - (reply: 10)
  273. detecting transmembrane protein exoression for monoclonal ab production - (reply: 1)
  274. antibody question - (reply: 7)
  275. spleenocyte count for hybridoma production - (reply: 1)
  276. can antibodies be added to cell culture for 10d before stain - immunocytochemistry (reply: 1)
  277. What can I do with blood sample from immunized rabbit? - we want to know whether there's antibody or not. (reply: 5)
  278. someone has frozen my antibody, is it still alive? - (reply: 2)
  279. Co-IP with two polyclonal antibodies - (reply: 2)
  280. Using denaturing protcol for his-protein purification - Can it still be used for antibody production????? (reply: 4)
  281. Secondary Antibody Choice - (reply: 6)
  282. making a monoclonal from scratch... - (reply: 1)
  283. which company can raise good antibodies? - raising antibodies using the native protein (reply: 3)
  284. using two primary antibodies for WB? - (reply: 3)
  285. Western Blot - unspecific antibody binding - (reply: 9)
  286. reducing background in western... - ...by blocking the antibodies (reply: 2)
  287. Secondary antibody - need to strip blot? - (reply: 4)
  288. Which bleed gives best result for antibody production? - (reply: 2)
  289. primary antibody for neuron - (reply: 6)
  290. Using Western Antibodies for IHC - General rules- suggestions (reply: 3)
  291. What kind of antibodies to use? Conjugated vs. unconjugated? - (reply: 19)
  292. Storage of antibodies - (reply: 3)
  293. antibody array for histone modification identification - (reply: 2)
  294. Purified protein (his-tagged) multiple bands on gel - all picked up by antibody - what could they be? (reply: 2)
  295. Detecting phosphorylated PKR - antibody and positive control - (reply: 4)
  296. Problem with Annexin FITC antibody - (reply: 5)
  297. Sandwich ELISA antibodies - (reply: 3)
  298. Can antibody recommended for ELISA or WB be used on IHC? - (reply: 5)
  299. Does HIV antibody kill HIV? - (reply: 6)
  300. solubility and stability of monoclonal antibodies - (reply: 2)
  301. Selecting epitomes of antibodies to apply in Western Blotting - (reply: 1)
  302. Standard Antibodies Against Separated Cellular Preps - (reply: 2)
  303. Array antibodies onto membranes - (reply: 4)
  304. Anti-FLAG antibody working for Western blot - I unsucessfully tried many different (reply: 4)
  305. Two different antibodies necessary for sandwich ELISA? - (reply: 3)
  306. why some antibodies need overnight incubation for Western blot - (reply: 4)
  307. aminocoumarin immunoprecipitation - looking for primary antibody (reply: 1)
  308. antibody inactivation of peptide - ELISA troubleshooting (reply: 1)
  309. Monoclonal antibody production - no cells show high O.D. - (reply: 8)
  310. Anti-PolyHistidin antibody - Best His6 antibody for western (reply: 2)
  311. Antibody for Sialic Acid residues - (reply: 3)
  312. Coupling Antibodies to alkaline phosphatase - (reply: 2)
  313. monoclonal antibody - use of HT (reply: 2)
  314. monoclonal antibody production - cell death in few wells (reply: 2)
  315. Polyclonal Ab versus Monoclonal Ab for immunoaffinity chromatography - (reply: 1)
  316. hybridoma cell line - (reply: 1)
  317. Western Blotting detection Antibodies........... - Are anti-sheep and anti-goat HRP interchangable? (reply: 2)
  318. Will antibody need any buffer to make link with bead - Which buffer should I use to link bead with antibody (reply: 1)
  319. Bacterial antibodies as the controls for Western - (reply: 1)
  320. Monoclonal Ab is always from mice? - (reply: 3)
  321. Antibodies to estrogen receptor, recommendations? - (reply: 1)
  322. Any experieces with unstable primary antibodies? - still recognizes recombinant protein but not native... HELP (reply: 1)
  323. recommendation on mitochondria matrix protein antibody? - (reply: 1)
  324. two mouse antibodies can be stained with different secondaries? - different colors? (reply: 3)
  325. PARP antibody working condition - (reply: 3)
  326. stability of hybridoma cell lines - how to produce totally stable hybridoma cel line? (reply: 3)
  327. Who sells good primary antibodies for Westerns? - Who's your favourite supplier? Least favourite? (reply: 24)
  328. One Antibody in Western Blot - (reply: 4)
  329. how can i preserve the purified mAb for a long time? - (reply: 7)
  330. What epitope is my antibody recognizing? - (reply: 3)
  331. Antibody Position on ELISA plate - (reply: 7)
  332. immunoprecipitation and western blotting assay by the same antibody - (reply: 4)
  333. Problems with protein expression - Protein expression, Antibody production (reply: 2)
  334. no cross-reactivity at 81% homology using polyclonal antibodies? - (reply: 3)
  335. polyclonal antibody doesn't pick up my protein during western!? - (reply: 4)
  336. How to make an antibody against my protein - (reply: 4)
  337. Antibody Vs. silver - (reply: 1)
  338. Fab Antibody - (reply: 4)
  339. Antibody source? - canine antibodies (reply: 1)
  340. alpha2 gabaa antibody for mice please! - (reply: 1)
  341. how many antibody eveyone use for CHIP assay - (reply: 2)
  342. Question on the recommended application of primary Antibody - (reply: 2)
  343. Co-IP experiments, Ratio of amount of cells/lysis volume/ug antibodies - (reply: 2)
  344. Purified antibodies in Western. - How can I find out how much to use? (reply: 12)
  345. Immunoaffinity chromatography-help - protocol for coupling of antibodies (reply: 2)
  346. Rabbit monoclonal antibody produce - (reply: 1)
  347. Caco-2 Vs Hybridoma cells - contamination of Caco-2 cell line with hybridoma cell line (reply: 3)
  348. Amastigote of Leishmania-ELISA - Detaching of bound antibody (reply: 1)
  349. How much Antibody did I get? - Protein G column (reply: 1)
  350. Creatine Kinase antibody problem. - (reply: 3)
  351. Nuclear staining - staining with a non-nuclear antibody - (reply: 1)
  352. FACS analysis of receptor surface expression using fluorescent antibodies - (reply: 3)
  353. western blot secondary antibody incubation time - will long incubation cause high background? (reply: 3)
  354. question about antibodies & protein G/A sepharose in CO-IP - (reply: 6)
  355. Low PCR product for antibody gene - (reply: 4)
  356. very strong secondary antibody background - (reply: 7)
  357. Choice of colour of secondary antibody for IHC - (reply: 2)
  358. Antibody left overnight at room temperature - still usable? - (reply: 5)
  359. Two antibodies on one western Blot? - (reply: 4)
  360. Big DNA contamination in antibody fraction, HELP ! - (reply: 1)
  361. Creative ways to get my hands antibodies - ...for free (reply: 35)
  362. antibody storage in skim milk - I want to know % of sodium azide (reply: 8)
  363. Antibodies of different class in blood of different species. - (reply: 3)
  364. Reuse primary antibody - (reply: 4)
  365. non IgG secreting hybridomas - (reply: 7)
  366. How many antibody should be added for Immunoprecipitation - (reply: 7)
  367. Antibody with sodiem azide added to mammalian cells - (reply: 2)
  368. Mouse Monoclonal Ki-67 ? - (reply: 1)
  369. Getting my primary antibody for Western - (reply: 6)
  370. antibody generation - big problem: PLEASE HELP (reply: 1)
  371. antibody production - animal free systems? (reply: 3)
  372. How to choose an antibody? - for western or other applications (reply: 1)
  373. high antibody binding non specifically - (reply: 3)
  374. confuse about which antibody to use as a first antibody - (reply: 2)
  375. Non-specific binding of secondary antibodies in IF - Secondaries stain Swiss 3T3 nucleoli (reply: 1)
  376. proteins to raise the antibody againest to HIV - proteins (reply: 1)
  377. Role of buffer in antibody purification - (reply: 3)
  378. HELP: transfection reagent for suspension cell - mouse hybridoma siRNA transfection (reply: 2)
  379. How to conjugate the antibody to the beads. - (reply: 4)
  380. Custom antibody advice (which company ?) - (reply: 2)
  381. Same antibody for immunohistochemistry and immunocytochemistry - SAME ANTIBODY FOR IHC AND ICC (reply: 1)
  382. Western Blots - Monoclonal or Polyclonal Antibody...? - Which one to use? (reply: 1)
  383. Antibody - (reply: 1)
  384. Background with chicken primary antibody - (reply: 2)
  385. Protein Purification - Advice - dialysis/desalting/concnetrating and antibody production? (reply: 1)
  386. Western blot background and specificity - How good is my antiserum? (reply: 1)
  387. ...the best antibodies? - (reply: 10)
  388. antibody purification - (reply: 5)
  389. Anti-His tag monoclonal antibody - (reply: 3)
  390. Antibody adsorption protocol - Immunology (reply: 1)
  391. conservation of antibodies! - after purification! (reply: 5)
  392. 2 antibodies from same host - same host ab's in sandwich (reply: 4)
  393. Hybridoma Cell lines - How to do it (reply: 1)
  394. antibody in SDS-containing solution - (reply: 3)
  395. Dnmt1 antibody - (reply: 1)
  396. cpti antibody - (reply: 3)
  397. p85 antibody from Santa Cruz - (reply: 6)
  398. purification of Anti-p24 antibody - purification of Anti-p24 antibody (reply: 1)
  399. Antibody testing - what if antigen is not available in a soluble form? (reply: 2)
  400. On antibody storage and ChIP - (reply: 3)
  401. Help with purification of antibody against a GST-fusion - Antibody purification (reply: 2)
  402. Self-purified antibody - viability and quantity problem - (reply: 1)
  403. Incubating the filters two overnights with primary antibody. - WESTERN BLOTTING (reply: 3)
  404. Antibody Viability - are my antibodies alive?? (reply: 3)
  405. How are antibodies typically injected into living systems? - (reply: 6)
  406. growing hybridomas - (reply: 1)
  407. CS antibodies - (reply: 5)
  408. Tissue staining problem with polyclonal antibody - (reply: 3)
  409. Looking for ELISA kit for proteinase 3 - Not anti-proteinase 3 antibody ELISA kit (reply: 1)
  410. anyone has idea to raise antibody, how much protein need? - (reply: 1)
  411. why did precipitate formed after antibody freez/thaw ? - (reply: 2)
  412. antibody problems in western - Got similiar western signals for three different p (reply: 8)
  413. Freezing method for Hybridoma - (reply: 1)
  414. what is the role of glycine in antibody purificiation??? - (reply: 3)
  415. Which antibody to use for ChiP? - Monoclonal or polyclonal? (reply: 5)
  416. Antibody for inflammatory cells - (reply: 2)
  417. Source for PAR antibody? - IHC for Poly-ADP-Ribose on rat tissues (reply: 2)
  418. EPEC Antibody - Need commercial source of EPEC Antibody (reply: 1)
  419. My antibody recognizes two bands - I tried everything and I have still two bands (reply: 2)
  420. Alkaline phosphatase cnjugated antibody - secondary antibody (reply: 5)
  421. antibody clones? - (reply: 2)
  422. Immunoprecipitation - getting rid of antibody heavy/light chains (reply: 6)
  423. Antibody Substitutes - (reply: 1)
  424. Reliable vendor that sells small aliquots of Antibodies? - (reply: 1)
  425. Immunoprecipitation - removing antibody? (reply: 4)
  426. Can antibodies and proteins be vortexed? - (reply: 4)
  427. Recycling antibodies in confocal microscopy?? - (reply: 2)
  428. what company make good antibody - (reply: 2)
  429. How many times do you reuse a primary antibody? - (reply: 7)
  430. Help!! a-tubulin antibody did not work...... - (reply: 1)
  431. Difference between polyclonal and monoclonal anitbodies - (reply: 2)
  432. Monoclonal antibody but getting multiple bands??? - (reply: 3)
  433. Help on ELISA antibody binding principles - (reply: 2)
  434. anti-rodent secondary antibody - (reply: 2)
  435. phospho-(Ser) PKC substrate antibody - (reply: 1)
  436. hybridoma cell question - (reply: 4)
  437. Need some antibodies - (reply: 2)
  438. Reuse antibodies for further incubation in Western blot - (reply: 5)
  439. phospho antibody detect untreat lane - (reply: 6)
  440. antibodies to epitope tags - (reply: 1)
  441. p16 antibody in western blotting, weird results - western blotting, p16 (reply: 4)
  442. Western blot detection other than antibody? - (reply: 5)
  443. Phospho-Elk1 specific Antibody...which band?? - (reply: 2)
  444. Size of Antibodies - (reply: 1)
  445. Multiplex Cytokine Assays - Antibody coupling - (reply: 3)
  446. monoclonal antibody production - monoclonal antibody production (reply: 3)
  447. online database for antibodies - (reply: 3)
  448. phospho-specific antibody - (reply: 1)
  449. Immunoprecipitation - peptide antibody (reply: 2)
  450. custom antibody synthesis - (reply: 1)
  451. anti mouse BCRP1 - polyclonal antibodies (reply: 1)
  452. phosphospecific antibody - how to get phosphospecific antibody (reply: 3)
  453. production of monoclonal antibodies - (reply: 2)
  454. Antibodies: polyclonal vs monoclonal - (reply: 2)
  455. western blotting with anti-goat HRP - bands after ECL but only secondary antibody (reply: 1)
  456. an anti-goat-HRP conjugate secondary antibody? - an anti-goat-HRP conjugate secondary antibody? (reply: 4)
  457. antibodies against membrane proteins - detection failure (reply: 3)
  458. multiple bands from monoclonal antibody - (reply: 1)
  459. antibodies against methyldeoxycytidine? - (reply: 1)
  460. Western Blot after Coomassie stain of antibody? - Western Blotting after Coomassie stain? (reply: 3)
  461. antibody shelf life? - (reply: 2)
  462. Hybridoma stability - Hybridoma (reply: 19)
  463. antibody to EGFP - anti-EGFP (reply: 6)
  464. Polyclonal vs. Monoclonal antibody for Sandwich ELISA? - (reply: 10)
  465. SDS PAGE - Validate method for MAb purity test (reply: 3)
  466. Antibodies using Recombinant proteins as antigen - (reply: 3)
  467. antibody stability - (reply: 2)
  468. Antibodies to recombinant proteins - Any advice on production of proteins? (reply: 2)
  469. Antibodies to Recombinant Proteins - Advice on antibody production?? (reply: 1)
  470. antigen / antibody immobilization - (reply: 1)
  471. how to do the antibody supershift through PAGE gel - (reply: 2)
  472. SOD1 (Cu/Zn) ANTIBODY ? - (reply: 1)
  473. Antibody against XanthineOxidase - (reply: 1)
  474. Murine MAb IgG purification - monoclonal antibody purification from ascites & supernatant (reply: 1)
  475. Monoclonal antibodies on MOUSE Tissue - Monoclonal antibodies on MOUSE Tissue (reply: 2)
  476. Cloning antibodies - (reply: 1)
  477. von Willebrands primary antibody - (reply: 1)
  478. Hybridoma screening problem - (reply: 1)
  479. hybridomona production - (reply: 2)
  480. Insitu antibody staining - (reply: 1)
  481. MHC Class I Western blotantibody??? - (reply: 1)
  482. Annti-Rad51/RecA antibodies - (reply: 1)
  483. labeling antibody withfluorescein - (reply: 1)
  484. how to reserve  antibody ofNOS? - (reply: 1)
  485. ELISA incubator - need an incubator or oven for capturing antibody (reply: 1)
  486. double label immunocytochemistry - protocol for antibodies from same animal (reply: 1)
  487. HSV-TK antibody - (reply: 1)
  488. how to generate antibody? - (reply: 2)
  489. His-tag Westerns: Bestcommercial antibody - (reply: 1)
  490. crosslinking of monoclonal Absto Protein G (or A) -Sepharose - (reply: 1)