Good GFP antibody from SANTA CRUZ for Western Blotting? - (Jul/20/2007 )

Got a couple (3) constructs with GFP tags and was wondering if any of you know of a great, clean (monoclonal??) anti-GFP from Santa Cruz that would work well for tagged proteins in westerns? It would be helpful if the antibody recognized both GFP AND EGFP and as a bonus work well in Immunohistochemistry. I know that's a lot to ask for from one antibody, but one can hope

-MarkER-

QUOTE (MarkER @ Jul 20 2007, 02:11 PM)

I routinely use sc-9996. It's a monoclonal which, at least in my hands, works great for westerns and fairly well for IF (cells!) but I'm not sure how it'll be for tissue samples. There is a bit of background with IF but nothing extreme.

-rkay447-

A good antibody from santa cruz? Nuff said

-DLY-

QUOTE (DLY @ Jul 23 2007, 06:47 PM)

A good antibody from santa cruz? Nuff said

Actually, the sc-9996 (or we usually call the B2 antibody) is OK. It works, although not as sensitive as other available anti-GFP Abs, but it did work, with minimal background. For WB is acceptable. IF is OK. I also don't know about tissue. IP: it pulls down GFP-tagged proteins, but with not as high efficiency as others.

-Almasy-

I would suggest that you also have a look for the antibody with companies other than SantaCruz e.g. Invitrogen's molecular probes!!

-repeatcell-

I could imagine that abcam also have what you are looking for! I also agree with repeatcell, molecular probes is great!

-DLY-

Does anyone also have an idea if antiodies again YFP are also available. Or does antibodies against GFP also recognize YFP on western blots?

-repeatcell-

The Clontech Ab (632460) living colour full-length rabbit antibody work for all living colours that are modifications from GFP, including YFP.

-Almasy-