Can anyone recommend the best FLAG and c-myc antibodies for co-IP? - (Feb/13/2007 )
I am in the process of setting up co-IP for protein-protein interaction in plants. I tagged one protein with 6xmyc and the other with FLAG. Can anybody suggest some good antibodies?
Thanks,
Baoxiu
-Baoxiu-
For flag tag you might try anti-flagM2 from sigma.
Good luck!!!
karmen
-karmen-
QUOTE (karmen @ Feb 14 2007, 04:09 PM)
For flag tag you might try anti-flagM2 from sigma.
Good luck!!!
karmen
Good luck!!!
karmen
abcam has very good c-myc Ab
but very expensive
-Katja W.-
i agree with carmen for flag antibody. agarose m2 is very good in my hands and ver reproductive
-fred_33-
QUOTE (Baoxiu @ Feb 13 2007, 07:51 AM)
I am in the process of setting up co-IP for protein-protein interaction in plants. I tagged one protein with 6xmyc and the other with FLAG. Can anybody suggest some good antibodies?
Thanks,
Baoxiu
Thanks,
Baoxiu
I have had good results with the Stratagene M2 clone as well for FLAG.
-viper-