HA antibody - (Mar/29/2008 )
Hey everyone.
I am doing this IP using a HA tagged protein but the problem is that my HA ab is not great for IP but really good for Western blots. Does anyone have an HA antibody that is good for IP. I started using the abcan goat-HA and it is ok but not great. I bought the Santa cruz one and i am in the process of testing it. does anyone have any suggestions about a good HA ab?
thanks
-funkyscientist-
QUOTE (funkyscientist @ Mar 29 2008, 09:31 AM)
Hey everyone.
I am doing this IP using a HA tagged protein but the problem is that my HA ab is not great for IP but really good for Western blots. Does anyone have an HA antibody that is good for IP. I started using the abcan goat-HA and it is ok but not great. I bought the Santa cruz one and i am in the process of testing it. does anyone have any suggestions about a good HA ab?
thanks
I am doing this IP using a HA tagged protein but the problem is that my HA ab is not great for IP but really good for Western blots. Does anyone have an HA antibody that is good for IP. I started using the abcan goat-HA and it is ok but not great. I bought the Santa cruz one and i am in the process of testing it. does anyone have any suggestions about a good HA ab?
thanks
I believe that I've used the santa cruz one in the past with sucess.
-smu2-