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antibody purification - how to purify from serum free medium (Nov/28/2006 )

Hi everybody,
Can someone please tell me how to purify monoclonal antibodies from Protein Free Hybridoma medium (PFHM) (GIBCO) using protein G column. Can it be purified same way as is used to purify from cell culture supernatant.
Thanking you in advance
Leelaram

-leelaram-

QUOTE (leelaram @ Nov 28 2006, 12:38 PM)
Hi everybody,
Can someone please tell me how to purify monoclonal antibodies from Protein Free Hybridoma medium (PFHM) (GIBCO) using protein G column. Can it be purified same way as is used to purify from cell culture supernatant.
Thanking you in advance
Leelaram


Yes.

-Minnie Mouse-