Freezing method for Hybridoma - (Jul/28/2005 )

Hi,
I am generating hybridomas from transgenic mouse spleen inorder to analyse the repertoire (Light chain). I ended up having more than 300 master clones (and many more to come) from my fusions and i am not able to freeze all of them in conventional way since space is limited. I was wondering is there any freezeing method available in 96 well format or any other method which can occupy less space in liquid nitrogen tank. I would greatly acknowledge if anyone has such technology to do it.

thanks in advance
Guru

hi maybe you can try to add freezing medium on your plates, and bring it at -80°. ou can keep these for month at -80. But in liquid nitrogen you must use a closed vial and 96well plates are not ok for that...
Depends when you will need to test your clones...
hope that helps.
Fred