Co-immunoprecipitation - Source of cheap irrelevant antibody (Dec/02/2008 )
Hi,
I have just started to do co-immunoprecipitations and my protocol tells me to pre-clear my cell lysates with an irrelevant antibody of the same isotype. This is fine except antibodies are so expensive and some protocols want me to put up to 50 ug in!! Does anyone know of a source of cheap mouse antibody? It doesn't matter what its raised to as I'll throw it out after the pre-clearing step any way,
Thanks,
P
-Penguin-
QUOTE (Penguin @ Dec 2 2008, 09:10 AM)
Hi,
I have just started to do co-immunoprecipitations and my protocol tells me to pre-clear my cell lysates with an irrelevant antibody of the same isotype. This is fine except antibodies are so expensive and some protocols want me to put up to 50 ug in!! Does anyone know of a source of cheap mouse antibody? It doesn't matter what its raised to as I'll throw it out after the pre-clearing step any way,
Thanks,
P
I have just started to do co-immunoprecipitations and my protocol tells me to pre-clear my cell lysates with an irrelevant antibody of the same isotype. This is fine except antibodies are so expensive and some protocols want me to put up to 50 ug in!! Does anyone know of a source of cheap mouse antibody? It doesn't matter what its raised to as I'll throw it out after the pre-clearing step any way,
Thanks,
P
Isotype selection depends upon what primary mouse antibody you are going to use.
If it is IgG (most likely), get normal mouse IgG from santacruz. You don't need to go into details like IgG1 or IgG2a for IP purpose. That may be important for things like flow and ELISA.
-cellcounter-
QUOTE (cellcounter @ Dec 3 2008, 06:15 AM)
Isotype selection depends upon what primary mouse antibody you are going to use.
If it is IgG (most likely), get normal mouse IgG from santacruz. You don't need to go into details like IgG1 or IgG2a for IP purpose. That may be important for things like flow and ELISA.
If it is IgG (most likely), get normal mouse IgG from santacruz. You don't need to go into details like IgG1 or IgG2a for IP purpose. That may be important for things like flow and ELISA.
Thanks! Have found 200ug mouse IgG for only £17!!
(my boss will be happy)
-Penguin-