how to label an antibody - (May/21/2008 )

Can anyone give me a procedure for labeling an antibody with a fluorochrome? Thank you so much

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you can check piercenet.com for info on selecting fluorophores and reactions. They also have kits to make your life real easy.

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Also the Alexa kits from Molecular Probes. Typically, you need 100 ug of protein, although there is a microscale labeling kit that works with as little as 20 ug, if I remember correctly.

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does anyone know the mechanism behind this kits? I need to know how this works

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