someone has frozen my antibody, is it still alive? - (Oct/20/2006 )

i've received many materials for imunnoprecipitation- one of them, an antibody, was put by mistake in the -20c freezer.
when i came back from a long vacation (~1 month) i found it and in the product details it was written:" do not freeze". it should have been in 4c.
what do you think, is there any hope or is it lost?
thanks
sybil

---

if it was shock freezen before storage at -20°C it had been better than slow cooling down to -20°C; if it is an commercial Ab, there may be a datasheet about the buffer components; salt, glycerol, sucrose, stabilizers (proteins) should protect from freeze damage; nevertheless, you have to try; I think the chance that it still works is not too bad...

---

i dont think it should matter so much, as most of the antibodies we have bought advised us to store in 4C. But we freeze them not in -20C but -80C. And they all work efficiently.

so give it a try with a positive control if u r worried. but I am confident it will work nicely.

---