What is needed for a hybridoma growth culture - (Jun/04/2008 )
Good morning,
I have a frozen culture of 12CA5 Hybridoma. I have not been able to find a protocol that contains the ingredients I have been told need to go in the culture.
DMEM, FBS, 2BE, these i have found in a protocol, however, I have no mention of how much essential and non-essential amino acids should be add to the complete media.
thank you for your help
Jonathan
-weinstej-
QUOTE (weinstej @ Jun 4 2008, 10:43 PM)
Good morning,
I have a frozen culture of 12CA5 Hybridoma. I have not been able to find a protocol that contains the ingredients I have been told need to go in the culture.
DMEM, FBS, 2BE, these i have found in a protocol, however, I have no mention of how much essential and non-essential amino acids should be add to the complete media.
thank you for your help
Jonathan
I have a frozen culture of 12CA5 Hybridoma. I have not been able to find a protocol that contains the ingredients I have been told need to go in the culture.
DMEM, FBS, 2BE, these i have found in a protocol, however, I have no mention of how much essential and non-essential amino acids should be add to the complete media.
thank you for your help
Jonathan
Jonathan,
See if you find what you are looking for here..
Hybridoma culture: http://search.vadlo.com/b/q?sn=158621799&a...lture&rel=0
Hybridoma medium:http://search.vadlo.com/b/q?sn=158621799&a...edium&rel=0
..
-cellcounter-