Specifity of antibodies - (May/07/2008 )

Hi friends,
I have a very basic question about antibodies in western blot and also immunofluorescent microscopy. Does an antibody for total ERK binds to phosphorylated ERK as well?

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most likely.

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antibodies are certified for successful usage in IB or IF or both; if not you have to try; if your non-phospho Erk-Ab is not directed to one of its numerous phosphorylation sites, there should be no perturbation by phosphorylation of Erk

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