Western Blot after Coomassie stain of antibody? - Western Blotting after Coomassie stain? (Aug/10/2004 )

Is it possible to do Western Blotting after Coomassie staining the mb?
Cheers

I assume you mean Coumassie the gel...

If so, I've used Invitrogen's simplyblue (a great stain). It comes straight out of the gel in the methanol used for western transfer (typically 10-20%)

Does this help?

Dicky