antibody question - (Nov/01/2006 )

hi every one
i have an antibody dilution question. i receieved my antibody as a powder. 100ug/ 1mg/ml IGG i am not sure what this means i need to dilute it and dont know how much water/pbs to add.i use 1ug for my application please help

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could it be that you have 100 µg and they recommend to resuspend to a final concentration of 1mg/mL ?

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I think missele is right; what is irritating is the first slash in the notion "100µg/ 1mg/ml"
is it really written in that way? is it hand-written as a gift or on a company-made label?

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hi
this is what the label on the antibody says: its from a company.

Size: 100 µg
Clone:
Host / Isotype: Rabbit
Immunogen: Purified GST:SF-1 fusion protein.
Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 ug of epitope affinity purified IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide.

i need to use 1ug/ml for my system. how do i dilute this powder and how much water do i add?
please reply asap

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the label makes it seem as if it is a solution and not a powder.

if it is a powder then suspend it to 100 ul (you can just add 100 ul of water). this will put everything back to the concentrations given on the label.

then use 1 ul/ml of your system.

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mdfenko is right, resuspend in 100 µLwater. you will get a 1 mg/mL of purifed IgG in PBS containing 1mg/mL BSA and 0.05% azide.

in fact the true is that they prepared a 1mg/mL solution, in PBS containing 1 mg/mL BSA as protein carrier and 0.05% sodium azide as preservative.
they lyophylsated 100µL and that's what you have.

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a senior person in my lab said resuspend in 1ml. which gives 100ug/ml=0.1ug/ul and to use 10ul for my experiment, which would make it 1ul/ml is this wrong i am completely confused please help me.

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personnally, I would dilute in 100 µL of water, to get 1 mg/mL in PBS 1X.
Then I would take an aliquote (let's say 10 µL) that I would dilute 10 times (by adding 90 µL of PBS), and then use 10 µL for my experiment.

If you resuspend your powder in 1 mL you will dilute the PBS, and dilute the protein carrier. It will be less stable.

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