primary antibody for neuron - (Oct/03/2006 )

hi...
i'm culturing rats neural stem cells and will identify the differentiate cells through immunocytochemistry. can anybody suggest what is the primary antibody to be use?
thanks....

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look for something like tau, NeuN or btubulin. this should be neuronal markers (in mice, so you have to check anyway).

dedee

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Doublecortin (DCX)

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Dedee is right, but I would like to add more important marker, GFAP or GLAST , a glia marker. Because your cells can differentiate both neuron or glia, right?

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thanks kobrain.
But I'm quite confius between marker and antibody. As i know, theres a lot of marker for neural stem cells like GFAP, Nestin, Anti B tubulin and so on. But I don't know what is the primary antibody to detect them.
thank 4 helping me.

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Tuj is what we check for..nestin too...

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Actually, until now there is no primary antibody to detect them. You have to combine these markers to determine your study. The more you test on these specific marker (glial/neuron), the better evidence you get in your result!
Hope this help a little!

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