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Transfection Related Discussions
  1. Some-what large scale transfection - (reply: 2)
  2. Transient transfection trouble- massive cell death - (reply: 1)
  3. selection of transfected cells - (reply: 1)
  4. Stable transfection problem - Problem with pBM vector and Phoenix packaging cells (reply: 3)
  5. too few cells used for electroporation/ transfection - (reply: 4)
  6. suitable vectors for 3' UTR Luciferase? - (reply: 2)
  7. cotransfection selection plasmid - need antibiotic marker rather than B galactocidase (reply: 1)
  8. what passage is too high for transient transfection? - (reply: 1)
  9. Toxicity of Transfection -> Endotoxin? - (reply: 4)
  10. Transfected copies per cell - (reply: 1)
  11. naked shRNA transfection - (reply: 2)
  12. Troubles in transfection methods! - All cells die before making a selection (reply: 5)
  13. DsRed stable transfections? - problems with Stoplight vector in Flip-in cells (reply: 4)
  14. Working out PEI:DNA ratio for transfection - (reply: 2)
  15. Luciferase Assay - (reply: 2)
  16. reproducibility of luciferase assay - (reply: 1)
  17. Advise for transfection efficiency - (reply: 1)
  18. Made an mistake during transfection! - Need to know if I corrected it by doing what I did. (reply: 2)
  19. transfection - (reply: 1)
  20. Transfection of HKC-8 - (reply: 1)
  21. 293T cells adhesion and transfection... - Looking for cell line for localization studies... (reply: 3)
  22. weird bacterial contamination following transfection - (reply: 2)
  23. Transfection in macrophage - It is possible?? (reply: 2)
  24. H9 Cell transfection - H9 cell transfection protocol (reply: 4)
  25. Cotransfection of DNA and siRNA in Neuro2a cells - (reply: 1)
  26. Need help with transfection! - GFP + HSP (reply: 3)
  27. CV1 transfection using Lipofectamine 2000 - (reply: 3)
  28. Glycerol shock or chloroquine? - CaPO4 Transfection Optimization (reply: 2)
  29. Transient Transfection into HEK -> EL expression - (reply: 2)
  30. Cell growth inhibition by lipofectamine 2000 - (reply: 1)
  31. What is the most sensitive assay for promoter strength? - dual luciferase assay? GFP? SEAP assay? Miller or something else? (reply: 3)
  32. Luciferase reporter assay - (reply: 2)
  33. pNL43 transfection problem - (reply: 3)
  34. Transfection & Wound Healing Assay - (reply: 2)
  35. HEK293 tansfection with pcDNA-YFP - can anyone help?! - stable transfections (reply: 2)
  36. miRNA transfection concentraion issue - Question about transfection reagent ratio (reply: 1)
  37. Plasmid transfection - should amount be adjusted according to insert size? - (reply: 4)
  38. Possible transfect siRNA using Calcium Phosphete? - siRNA transfection (reply: 2)
  39. B-galactosidase variability in Luciferase assays - B-galactosidase variability in Luciferase assays (reply: 8)
  40. Is it a general effect that stably transfected cell line has lower cell prolifer - (reply: 4)
  41. Stable transfection of Pro-apoptotic genes - How is it possible? (reply: 4)
  42. plasmid transfection - (reply: 1)
  43. Daudi cells transfection - (reply: 1)
  44. low signals of luciferases in PPARg transfection - (reply: 2)
  45. HUVEC siRNA transfection help please - I need suggestions on what else to try! (reply: 3)
  46. test the knockdown effficiency with siRNA before transfection with lentivirus? - (reply: 1)
  47. suitable transfection reagents - (reply: 2)
  48. New to Transfections! Please Help! - What specifically is the DNA in my transfection protocol??? (reply: 2)
  49. After transfection and passaging I loose my virus - (reply: 4)
  50. siRNA transfection in MLE12 cells - (reply: 3)
  51. Transfect competent cell? - (reply: 1)
  52. CaPO4 transfection and Temperature - (reply: 1)
  53. Stable transfection of SHSY5Y - (reply: 1)
  54. % confluency question - when to transfect the cells? (reply: 2)
  55. Help...cell transfected with empty vector(control) don't retain fluorescence - PIRES2 (reply: 3)
  56. HOW TO MAKE A STABLE TRANSFECTED CELL LINE with COS-7? - (reply: 2)
  57. good transfection efficacy, no proteins in WB - the weirdest problem of my life (reply: 1)
  58. western blot problem - no expression on stable transfection. (reply: 9)
  59. Scaling up siRNA transfection - (reply: 1)
  60. scintillation counting for luciferase assay - (reply: 1)
  61. Lysis buffer for luciferase assay - (reply: 1)
  62. ProteoJuice Protein Transfection - (reply: 2)
  63. siRNA transfection: DNA conc optimization problem - (reply: 1)
  64. Transient transfection with a pcDNA plasmid - How long does it take for chromosomal integration??? (reply: 4)
  65. HEK293 vs HEK293T - which is better for transfection (reply: 2)
  66. Stable miRNA transfection - (reply: 7)
  67. stable transfection - (reply: 6)
  68. siRNA transfection in endothelial cells - (reply: 3)
  69. Luciferase assay and luminometer - Technical details (reply: 3)
  70. significance of confluence in transfection efficiency - (reply: 3)
  71. How to confirm whether the cell line was successfully transfected with two plasm - Co-transfection of two plasmids to 293 cells (reply: 6)
  72. prepare the SCLC for the transfection - SCLC cell seeding for transfection (reply: 4)
  73. Hepa1-6 transfection? - What do you use? What is/is not working? (reply: 2)
  74. double transfection plus siRNA - in HEK293 cells (reply: 1)
  75. calcium phosphate transfection questions - (reply: 1)
  76. Transfection of 293-T cells in 6-well plate, using PEI - (reply: 4)
  77. Need help with transient transfection - (reply: 4)
  78. Transfections questions - (reply: 5)
  79. Transfection of human fibroblasts - (reply: 5)
  80. siRNA transfection - (reply: 4)
  81. Cos stable transfection - (reply: 1)
  82. plasmid transfection in hepatocytes - (reply: 1)
  83. siRNA transfection in primary hepatocytes - (reply: 1)
  84. Calcium Phosphate transfection of HEK293 on poly-lysine plates - (reply: 3)
  85. Transfection of siRNA in suspension cells - (reply: 6)
  86. normalization of luciferase RLU data - (reply: 2)
  87. luciferase vector construction - (reply: 3)
  88. RAW 264.7 cell transfection - Probs transfecting RAW cells with lipofectamine LTX (reply: 2)
  89. FuGene 6 transfection problems - (reply: 15)
  90. Help with transfection of HEK cells with p53 flag - (reply: 5)
  91. Will GFP interferes with luciferase assay result? - (reply: 3)
  92. stable transfection ratio - (reply: 1)
  93. query about efficacy of calcium phosphate transfection - (reply: 8)
  94. transfection with ligation mix? - (reply: 6)
  95. stable transfected cell line in absence of G418 - (reply: 6)
  96. good control for transfections - (reply: 7)
  97. Protocol for transfection of mES using lentivirus - (reply: 3)
  98. HELP: Transfection of Huh7 - (reply: 5)
  99. luciferase assay - (reply: 3)
  100. Transfection using Calcium Phosphate - (reply: 9)
  101. Transient Transfection of HeLa cells. - (reply: 1)
  102. Transfection of B-cells - siRNA transfection in Primary effusion lymphomas (reply: 4)
  103. Can any one help to transfect these cells ? - SCN neuron transfection (reply: 2)
  104. good reagents for transfection of siRNA in Caco-2 - (reply: 1)
  105. Transfection of Drosophila cell culture - Which is the best transfecting agent? (reply: 1)
  106. Plasmid vs Lentivirus as a transfection vector - Opinions? (reply: 4)
  107. Transfection of L929 cells with Poly (IC) - (reply: 1)
  108. Fugene 6 transfection efficiency? - (reply: 12)
  109. BHK Cell Transfection - (reply: 2)
  110. Help: MIN6 Transfection 96well plate Lipo2000 - (reply: 10)
  111. polystyrene or polypropylene tubes during transfection - (reply: 1)
  112. siRNA "knock-out"/transfection "knock-in" - (reply: 10)
  113. does low plasmid concentration affect transfection rate? - (reply: 4)
  114. luciferase assay - How to adjusting parameters - (reply: 5)
  115. HELP: transfection reagent for BJ cells - (reply: 1)
  116. Search more efficient transfection reagent for BJ cells - (reply: 1)
  117. Search transfection reagent for BJ cells - (reply: 2)
  118. trobule with double stable transfection - (reply: 1)
  119. HepG2 death with INTERFERin transfection - (reply: 2)
  120. primary sertoli cell transfection - (reply: 2)
  121. Anyone else have toxicity issues with Lipofectamine 2000? - (reply: 6)
  122. Hygromycin-dependent selection of stable transfectants - (reply: 4)
  123. Lipofectamine LTX - (reply: 4)
  124. How to Transfect T cells - (reply: 2)
  125. Transfection - Something wrong with transfection? (reply: 4)
  126. Looking for a good cell line to study beta-catenin activity using transient tran - Please recommend a cell line that is good for transient transfection o (reply: 3)
  127. cos-7 cells transfection using calcium phosphate - (reply: 1)
  128. TRANSFECTION IN HEPG2 - (reply: 5)
  129. stage at which transfection should be done, when cells are round or flat adhered - (reply: 4)
  130. transient transfection - (reply: 4)
  131. Stable transfection with or without EGFP - (reply: 3)
  132. triple stable transfection - (reply: 7)
  133. tell me what im doing wrong? transfection - (reply: 8)
  134. Luciferase concentration question - (reply: 3)
  135. Dual Luciferase assay - What does pRLTK have? (reply: 1)
  136. lentivirus tranfection problem - (reply: 3)
  137. transfection reagent for HUVECs ? - high cytotoxicity problem (reply: 2)
  138. Transfection with DEAE Dextran - (reply: 1)
  139. siRNA transfection of TWO targets simultaneously - Any experience/advice? (reply: 1)
  140. help with stable transfection screen - (reply: 1)
  141. transfect from full plasmid and transfect from PCR product - (reply: 5)
  142. Clumping of normal colon cells due to transfection reagent - Counting problems (reply: 6)
  143. Elute DNA from Watmann paper - I received a plasmid in the mail and cant get transfectants (reply: 2)
  144. co transfection plasmid and siRNA - message from gmgabriel68 (reply: 1)
  145. Using Adenovirus to transfect cells - (reply: 5)
  146. How to improve transfection efficiency? - (reply: 2)
  147. cotransfection of CHO cells - (reply: 1)
  148. RAW cell line transfection - (reply: 2)
  149. what time is better to seed cells in well or dishes before transfection? - (reply: 7)
  150. Transfection of Vero cells - Nothing new but it simply didn't work...... :( (reply: 4)
  151. Single cell luciferase bioluminescence detection. Is it possible? - (reply: 1)
  152. Cell death with DMSO treatment, during transfection using polybrene - (reply: 2)
  153. stable transfection high expression - problems with low expression in stably transfected cells (reply: 1)
  154. Low transfection efficiency using PEI - I'm trying to transfect BHK-21, with a large size plasmid (13kb), (reply: 9)
  155. Effect of HEK293T passage number on transfection efficiency - (reply: 11)
  156. read a luciferase assay - value too high (reply: 3)
  157. Loss of expression of EGFP-actin from MCF-7 transfected with pEGFP-actin - (reply: 3)
  158. Passaging stable transfected cells after thawing - (reply: 4)
  159. Is that possible to establish a stable cell line which is transfected by an empt - (reply: 2)
  160. should I use transient or stable transfection - (reply: 4)
  161. pGL3 Luciferase assay - need experimental set up help (reply: 1)
  162. stable transfection problem - (reply: 1)
  163. Renilla Luciferase Assays - Wondered if anyone knew how to make the reagents "at home"?? (reply: 1)
  164. SiRNA Transfection in 60mm plates - (reply: 2)
  165. concentration of DNA for stable transfection with lipofectamine 2000 and the per - (reply: 7)
  166. HEK293 transfection using electroporation. Science or magic! - (reply: 2)
  167. Any prototol for stable transfection using Lipofectamine 2000? - (reply: 1)
  168. changed attchment of RMA/S cells after transfection, why? - (reply: 2)
  169. cell culture contamination - after transfection (reply: 15)
  170. T antigen status of human cells for transfection - (reply: 1)
  171. Protein-DNA Interaction by Deleting ENTIRE DNA-binding Domain then Transfection - (reply: 3)
  172. siRNA transfection - (reply: 5)
  173. Problems obtaining stable transfected 293 cells - (reply: 1)
  174. Luciferase assay-signal decrease - why this decay? (reply: 3)
  175. Stable transfection of H295R cells - (reply: 3)
  176. how to analyze transfection efficiency of miRNAs? - (reply: 3)
  177. tranfection of 3T3-L1 cells post differentiation - (reply: 2)
  178. transfected protein sequestered inside vesicles - (reply: 3)
  179. stable transfections using SCC 9 cells - (reply: 5)
  180. Stable transfection of human adrenocortical cancer H295R cell line - (reply: 1)
  181. the ratio of pGL3 promoter( control) vector in Dual luciferase assay - Dula luciferase assay (reply: 1)
  182. miRNA luciferase reporter assay queries - (reply: 1)
  183. Too Happy Cells - Cells are rapidly growing 7 affecting transfection (reply: 5)
  184. Hepg2 transfection - (reply: 4)
  185. Dual luciferase assay - (reply: 2)
  186. Can professional phagocytes be easilly transfected? - (reply: 3)
  187. Double - Triple stable transfection - (reply: 4)
  188. Stable transfection (How to examine my control clones) - (reply: 8)
  189. Luciferase Reporter Assay - Data analysis (reply: 2)
  190. mRNA induction after shRNA transfection - (reply: 3)
  191. HELP! About plasmid transfection in 96 well plate - (reply: 3)
  192. HEK 293 Stable Transfection and Selection Questions - (reply: 3)
  193. transfection agent -primary mouse macrophages, anybody? - (reply: 1)
  194. stable transfection for suspension cell lines - (reply: 1)
  195. siRNA transfection - (reply: 3)
  196. Why is an antibiotic free media necessary before transfection? - (reply: 4)
  197. cell culture with serum media and with serum free media and doubts in luciferase - (reply: 1)
  198. BHK cells transfection - (reply: 2)
  199. protocol for transfection(eletroporation) for HEK293 cell - (reply: 1)
  200. MigR1-GFP vector transfection - Did anyone transfect MigR1 using TransFast (reply: 4)
  201. TE diluted plasmids and transfection - (reply: 2)
  202. Transfection of RAW264.7 cells - Tips for increasing transfection efficiency? (reply: 1)
  203. co-transfection & transfection - (reply: 3)
  204. Transfection of cryopreservative cells - (reply: 3)
  205. Adenoviral Transfection - cells to check transfection efficiency (reply: 4)
  206. What are the main criteria for best transfection/infection? - (reply: 3)
  207. DEAE Dextran transiet transfections - (reply: 3)
  208. reporter vector cotransfection - (reply: 4)
  209. Stable transfection using plasmid with no selection gene - (reply: 2)
  210. hard to transfect cell lines - (reply: 1)
  211. transfection of chick cell - (reply: 3)
  212. Transient transfection of MEFs - (reply: 3)
  213. transfection of PC12 cell line - (reply: 1)
  214. siRNA + DNA transfection - (reply: 3)
  215. problems with transient transfections - (reply: 2)
  216. transfection with PA-1 cells - (reply: 1)
  217. transfection in neuron - (reply: 4)
  218. Transfection of T47D cells with FuGene in 10cm plates - (reply: 2)
  219. anyone has the recipe for the substrate of Rellina Luciferase Assay? - (reply: 3)
  220. triple-plasmids transfection - (reply: 2)
  221. Do cells transfected with lentivirus express specific surface markers - (reply: 3)
  222. DNA size related to the transfection efficiency？ - (reply: 9)
  223. using EGFP fluorescence as measure of transfection efficiency - (reply: 3)
  224. transfection of RAW 264.7 - (reply: 3)
  225. Do cells transfected with lentivirus express specific unique surface markers - (reply: 2)
  226. calcium-phosphate transient transfection efficiency? - (reply: 5)
  227. Transfection of HEK293 - 70-90% confluency of HEK293 (reply: 2)
  228. Transfection of expression vector for gel shift - how much vector should be transfected? (reply: 2)
  229. SL2 cell transfection - (reply: 1)
  230. HELP with stable transfection - (reply: 3)
  231. help: anenovirus transfection - (reply: 4)
  232. bad cell condition for transfection - (reply: 2)
  233. Any suggestions on triple transient transfection? - (reply: 2)
  234. Cytoplasmic granulation after siRNA transfection - (reply: 3)
  235. question about plasmid transfection - (water solution or buffer solution?) (reply: 3)
  236. retrovirus transfection in NIH3T3 - (reply: 3)
  237. a question about screening transfected cells by GFP protein - (reply: 5)
  238. After successful Transfection protein not detectable - (reply: 3)
  239. Stable transfection problem - About co-transfection (reply: 2)
  240. transfection in NIH3T3 cells - (reply: 10)
  241. Stable transfection utilizing ampicillin resistance? - (reply: 2)
  242. what transfection effeciency is needed for westen? - (reply: 2)
  243. Help! GFP transfection - (reply: 12)
  244. GFP siRNA transfection - readout - (reply: 5)
  245. Transfection of Breast Cancer Cell Lines - (reply: 2)
  246. Apply tranfection reagents DIRECTLY onto the cells. - After removing the medium. (reply: 4)
  247. PCR for checking transfection - (reply: 6)
  248. Transfection of SCG neurons. Anyone? - What method do you use? (reply: 5)
  249. siRNA transfection in suspended cell lines MV4-11, THP-1 - (reply: 1)
  250. about stable transfection - (reply: 2)
  251. calcium phosphate transfection tips. - (reply: 3)
  252. do you coat plates for HEK transfection? - (reply: 3)
  253. transfection problem...please help... - (reply: 6)
  254. linear tandem repeats of gene for transient transfection? - (reply: 3)
  255. Markers and selection in transfection? - (reply: 1)
  256. negative control expresse more luciferase activity than experiment in Luci assay - (reply: 6)
  257. RNA transfection VS Plasmid DNA transfection - (reply: 3)
  258. siRNA transfection problem - (reply: 4)
  259. COS-7 and PC-3 transfected with a GFP-AR fusion protein - (reply: 4)
  260. Used linear concatameric genes for transient transfection? - (reply: 2)
  261. Problem with transfection and protein expression - NO DIFFERENCE BETWEEN UNTRANSFECTED AND TRANSFECTED CELLS (reply: 10)
  262. what are selectable markers for stable transfection except gentamicin - (reply: 4)
  263. Selection of stable transfectants after transfection - (reply: 2)
  264. 12 well transfection - (reply: 5)
  265. siRNA transfection reagent - (reply: 9)
  266. beginners guide to stable RNAi transfection - (reply: 1)
  267. Calcium transfection problems - (reply: 3)
  268. Why serum-free during transfection? - (reply: 2)
  269. Can I select stable transfectants with amp-resistant vector? - (reply: 3)
  270. Basic transfection question - (reply: 12)
  271. transient transfection of a stable cell line? - (reply: 4)
  272. I want to inqure some questions about cell lines and cell phenotype - which cell line has the highest transfection efficiency as you know (reply: 1)
  273. have you ever used hygromycin to screen transfected HepG2 or gastric cancer cell - all the cells died, why? (reply: 1)
  274. Low transfection efficiency/protein expression - I've tried everything! (reply: 7)
  275. Plasmid CpG methylation after transfection - (reply: 2)
  276. Transfection and Expression: getting transcript but no protein expression - (reply: 3)
  277. Question on methods for transduction and transfection - (reply: 1)
  278. will linearizing plasmid prolong transient transfection ? - (reply: 1)
  279. The growth behaviour of the transfected cells in hygromycin selection media - (reply: 1)
  280. lipofectamine - role in transfection (reply: 7)
  281. Problems with plasmid transfection into primary keratinocytes - (reply: 3)
  282. How exactly does a stable transfection work? - Does replication of the plasmide happen? (reply: 7)
  283. Transient transfection on a stable cell line - (reply: 2)
  284. Dual Luciferase Assay -- Problems with reproducibility - (reply: 1)
  285. New Transfection technology (high efficiency) - (reply: 1)
  286. Coat Coverslips with Collagen IV/Transfect and Image PC12 Cells - (reply: 1)
  287. Transfection Toxicity lipofectamine2000 - (reply: 5)
  288. Split cells after siRNA transfection? - What to do when cells become confluent after transfection of siRNA (reply: 6)
  289. dual glo luciferase assay+ pRLTK transfection problem - (reply: 2)
  290. Transfection by electroporation of 293 suspension cells - electroporation of 293 cells for large scale transient expression (reply: 2)
  291. New to transfection field... can u help? - (reply: 4)
  292. Phenol/Chlorophorm extracted DNA - ok to use for transfection??? (reply: 2)
  293. Dual luciferase assay - (reply: 3)
  294. Stable transfection of large plasmid into breast cancer cells - (reply: 2)
  295. CIAP treatment for stable transfection ? - (reply: 1)
  296. use of miniprep DNA for transfection - (reply: 4)
  297. Hela cells and stable transfection help please! - (reply: 6)
  298. about MTS assay and plasmid transfection - (reply: 2)
  299. BALB/c 3T3 transfection - (reply: 2)
  300. transfection of dsRed - (reply: 2)
  301. HeLa transfection... - (reply: 3)
  302. transfection kit- any body knows? - (reply: 8)
  303. CHO suspension cells - adaptation to serum free suspension, and transfection using electroporation (reply: 3)
  304. selection of stable transfectants with ampicillin - (reply: 4)
  305. Selection Problems with G418 - CaPO4 Transfection and single cell picking (reply: 4)
  306. Flourescent-labelled substrate for luciferase detection - (reply: 2)
  307. transfection in MDAMB231 cells - (reply: 2)
  308. TF-bindingsite in luciferase - (reply: 2)
  309. luciferase reporter gene assay - (reply: 3)
  310. What's difference between RPMI and DMEM - which media I should use to culture cells after transfection? (reply: 3)
  311. Is it possible to do siRNA followed by transfection of dn of the protein? - HAs anybody tryed this?? (reply: 3)
  312. Transfection of 293 cells for protein expression - optimum conditions for transfection!!! (reply: 5)
  313. Why is non-viral transfection efficiency of endothelial cells low? - (reply: 1)
  314. transfection of NRK cells-lipofectamine toxicity - (reply: 10)
  315. SHSY5Y cell transfection - (reply: 5)
  316. Cell transfection - TransIT vs Fugen - (reply: 3)
  317. HeLa S3 transfection with microparticle loaded pDNA - (reply: 4)
  318. can the phoenix cell be transfected using lipofectamine ? - (reply: 2)
  319. question about transfection - (reply: 4)
  320. Lipofectamine transfection under the microscope - (reply: 7)
  321. Getting rid of the dead cells during selection of transfected colonies? - (reply: 2)
  322. selecting the transfected colonies after transfection - (reply: 1)
  323. optimizing selection of transfected cells - (reply: 2)
  324. cancer cell transfections - (reply: 2)
  325. help ! selecting transfected colonies - (reply: 2)
  326. transfection efficiency - how to calculate it? (reply: 4)
  327. low viability of stably transfected cell line from cryostock - (reply: 1)
  328. help in transfection of NHFs - (reply: 1)
  329. Stable transfection using pCMV-FLAG2 - has anybody done this? (reply: 3)
  330. Protein Transfection by Electroporation - (reply: 6)
  331. MIGR1 retroviral vector transfection - (reply: 3)
  332. Luciferase promoter design - (reply: 5)
  333. transfection and adenovirus - probability of having adenovirus (reply: 11)
  334. Does any one have a good resource for basics of transfection and transduction - (reply: 1)
  335. Antibiotic after transfection or not? - (reply: 2)
  336. Yeast contamination in a adenoviral transfection - (reply: 3)
  337. Low transfection of siRNA into HaCaT cells. Please help - (reply: 1)
  338. Help Frozen lipofectamine - (reply: 6)
  339. transfection of rat spinal cord neuronal cultures - (reply: 3)
  340. tools for transfection study - Other transfection tools? (reply: 1)
  341. transfection of HeLa cells - (reply: 4)
  342. I cannot get protein from my stable transfection cell - (reply: 4)
  343. Which is better! Lipofectamine or Fugene 6? - (reply: 9)
  344. Transfection - beginner - (reply: 3)
  345. SiRNA and GFP transfection MCF7 cells - (reply: 4)
  346. transfection of suspension cells - how to improve the transfection efficiency? (reply: 3)
  347. plasmid isolation from a transient transfection - (reply: 2)
  348. making stable clones through single cell dilution after transfection - (reply: 2)
  349. Lipofectamine Transfection of BAECs - (reply: 4)
  350. pcr product transfection help - (reply: 3)
  351. First time doing transfections! - please!!! some help or tricks!!!! (reply: 14)
  352. picked GFP transfected colonies do not retain flouresence after being picked - (reply: 3)
  353. Linealizated plasmid for stable transfection? - is that right? (reply: 1)
  354. Does it need cotransfection vector when retrovirus transfected hek293? - (reply: 7)
  355. Plasmid cotransfection for selection - (reply: 4)
  356. Help! transfection for Huh7 using Lipifectamine 2000 - (reply: 7)
  357. Transfection efficiency with RNAi - help (reply: 3)
  358. transfection of MCF7 cells - (reply: 2)
  359. Has anyone transfected ST15A cells? - Transfection of ST15A cells? (reply: 3)
  360. How to increase RNAi efficiency by siRNA transfection? - (reply: 4)
  361. cell death during transfection - (reply: 3)
  362. losing green light form pIRES2-EGFP transfected cells - (reply: 13)
  363. Has anyone transfected human primaries? - (reply: 5)
  364. Glioblastoma cell lines and transfection - stable transfection (reply: 5)
  365. transfection gfp visualization - (reply: 14)
  366. Time course for siRNA inhibition - When is the peak of inhibition after transfection ? (reply: 11)
  367. Endo-free DNA Preps, When? - For transfection?? For vaccines? (reply: 3)
  368. before transfection into mammalian cells first transfect into E-coli.... - (reply: 8)
  369. help with choosing model system for optimization of transfection of siRNA - and best deal for positive control? (reply: 5)
  370. Lipofectamine and FuGene 6 transfections - (reply: 33)
  371. RNAi of HepG2 cells - low transfection effeciency (reply: 1)
  372. stable 293 transfectants - (reply: 12)
  373. pcr product transfection - (reply: 8)
  374. Transfection of human skin fibroblasts - (reply: 13)
  375. stable or transient transfection method? - for protein expression (reply: 4)
  376. DLD1 transfection - (reply: 3)
  377. How to remove endotoxin? - want a endotoxin-free plasmid for transfection (reply: 5)
  378. Help ! with stable transfection - (reply: 3)
  379. how to avoid MEF when mES cells are transfected by lentivirus? - (reply: 4)
  380. pGL3 transfection and assay - tips for improvement? - improve on low luciferase values?? (reply: 1)
  381. Need protocol for mouse ES cell stable transfection - (reply: 1)
  382. improned transfection efficiency created by my labmate - (reply: 6)
  383. Antibiotic concentration for selection of transfectants - (reply: 7)
  384. I have a problem about the transfection of 293T - (reply: 2)
  385. Are transiently transfected DNA methylated in mammalian cells? - (reply: 3)
  386. Transient Transfection of 293T - (reply: 3)
  387. loss of expression in stable transfection - (reply: 4)
  388. why transfected cells has less RNA? - (reply: 2)
  389. Calcium phosphate transfection question - (reply: 4)
  390. Problem with shRNA stable transfection - (reply: 3)
  391. problem when doing transfection for the first time - (reply: 1)
  392. What is the best way to transfect MEFs? - and what medium do you use (reply: 7)
  393. PEI transfection of Human Cortical Neurons - (reply: 2)
  394. siRNA transfection of MIN6 cells - (reply: 2)
  395. Cells survived after G418 selection are transfected cells? - (reply: 2)
  396. Saos-2 cells transfection - (reply: 1)
  397. Triple stable transfection - (reply: 2)
  398. help with transfection of large size plasmid - (reply: 2)
  399. medium must be changed after Calcium transfection? - (reply: 4)
  400. How do you select for stable transfectants in a suspension cell line? Soft agar - (reply: 2)
  401. Dual Luciferase Assayproblem with cotransfection of reporters - (reply: 5)
  402. Transfection efficiency by GFP - (reply: 4)
  403. M17 cell transfection - (reply: 1)
  404. Calcium Phosphate Transfection - (reply: 4)
  405. retroviral transfection of pheonix cells - (reply: 3)
  406. how to build a stable transfection in a suspension cell - (reply: 1)
  407. Stable transfection selection with puromycin - (reply: 2)
  408. luciferase assay promoter - include the TATA box in promoter sequence? (reply: 1)
  409. Help! Ca Phos transfection not working - transfection (reply: 7)
  410. Transfection Efficiency - (reply: 14)
  411. Can I extract nuclear proteins in transfected cells? - (reply: 2)
  412. Transfection of inducible ER-fusion construct. - Estrogen in fetal calf serum? (reply: 5)
  413. Extensive cell death after transfection in a 24-well plate - (reply: 3)
  414. How to get dose depedent result from luciferase assay? - (reply: 1)
  415. Splitting transfected cells: do they still overexpress my gene of interest ? - (reply: 5)
  416. looking for a good siRNA transfection reagent for 3T3 L1 cells ? - (reply: 5)
  417. endothelial cells transfection - (reply: 2)
  418. transfection of M17 cells - (reply: 4)
  419. Why transfected neurons always die? - (reply: 1)
  420. Linear or unlinear plasmid before transfection for expression? - (reply: 2)
  421. How long DNA and siRNA stay in transfected cells - (reply: 2)
  422. Stable transfectants of HEK293 (selection with G418). Question about splitting ( - Cells growing confluent long before G418 kills them, is splitting smart or not? (reply: 9)
  423. RAW 264.7 cell line transfection - (reply: 1)
  424. transiently transfected vectors replication - do they replicate in an eukaryotic cell? (reply: 1)
  425. stable transfection - (reply: 3)
  426. stable transfection - (reply: 3)
  427. Transfection into a cell line which already express your protein - (reply: 3)
  428. problem transfection Hela cells - (reply: 8)
  429. How to know siRNA transfection efficiency? - (reply: 4)
  430. 96-well plate transfection using calcium phosphate method - (reply: 7)
  431. sterile DNA for transfection - (reply: 2)
  432. Do transfected vectors bind histones and/or methylate? - (reply: 10)
  433. plating cells for transfection - (reply: 2)
  434. Transient transfection on a stable cell line - (reply: 6)
  435. how to improve transfection efficiency? - (reply: 7)
  436. transfection or injection of siRNA - Which one is better? (reply: 9)
  437. direct transfection vs. retroviral transfection - (reply: 2)
  438. Transcient transfection of HaCaT cells - which is the most effective method? - (reply: 2)
  439. Oligofectamine Transfection - (reply: 2)
  440. I ran out of transfection agent...What to do? - (reply: 3)
  441. cellular death during dsRNA transfection. - (reply: 3)
  442. principle of transfection - wants to know the optimal time needed for transfection (reply: 2)
  443. Question about medium change during transfection - (reply: 1)
  444. Determining transfection efficiency - (reply: 2)
  445. stable transfection of mouse embryonic stem cells - help! (reply: 1)
  446. transfection with P/S or without P/S - help advise.. (reply: 4)
  447. Passive lysis buffer for luciferase assays - (reply: 11)
  448. Transfection of coronary artery endothelial cells - (reply: 1)
  449. Stem cell transfection - (reply: 2)
  450. transfection in eukaryotic cells - (reply: 1)
  451. HELP: transfection reagent for suspension cell - mouse hybridoma siRNA transfection (reply: 2)
  452. shRNA transfection: transient vs stable? - (reply: 3)
  453. DNA purity for transfection - (reply: 16)
  454. stable transfection of MDAMB231 and Malme3M cells. - (reply: 1)
  455. luciferase reporter vector - promega (reply: 1)
  456. renilla Luciferase and protein concentration - (reply: 1)
  457. TRANSFECTED 293 CELL FOR Luciferase assay - Dual Luciferase assay (reply: 8)
  458. transfection.. - help needed (reply: 2)
  459. transfection - (reply: 1)
  460. gene promoter - promoter luciferase (reply: 3)
  461. siRNA transfection reagent for primary cultured cells - (reply: 2)
  462. Trouble expressing a protein in transient transfection - (reply: 2)
  463. PBS causes cell death in transfection - (reply: 8)
  464. Double transfection - using Lipofectamine 2000 (reply: 6)
  465. Strange firefly/renilla luciferase behavior - (reply: 6)
  466. Transfection Reagents - (reply: 2)
  467. Please help me with SH-sy5y transfection - (reply: 1)
  468. expression of transfected gene - urgent (reply: 5)
  469. HeLa transfection - using Lipofectamine 2000 (reply: 20)
  470. cell transfection in 96 well plate - using lipofectamine 2000 (reply: 2)
  471. Supplier of Gencarrier transfection reagent - transfection reagents (reply: 1)
  472. Slit cells or add antibiotics, which is first after stable transfection - (reply: 8)
  473. what is the best way to set up the transfection? - (reply: 2)
  474. Can we purify Miniprep DNA and use for transfection? - (reply: 3)
  475. help: macrophages transfection and immunofluorescence - (reply: 2)
  476. Lipofectamine 2000 (Invitrogen) - How much can transient transfection expected ? (reply: 4)
  477. Duration of cell line transfected - (reply: 2)
  478. stable eukaryotic transfection. - Neomycine resistance and bad viral titer (reply: 1)
  479. Recommendation of transfection reagents - (reply: 5)
  480. siRNA Stable transfection in human fibroblast cells - (reply: 6)
  481. Double Transfection problems? - (reply: 1)
  482. transfection of Hek 293A cells - (reply: 5)
  483. Question for dual luciferace reporter assay - -need a reporter for transfection efficiency (reply: 5)
  484. transfection of primary cortical neurons - (reply: 2)
  485. Transfection of cells by viral DNA - (reply: 4)
  486. Transduction, Transfection, Transformation and Infection - (reply: 7)
  487. HELP: adenovirus transfection - (reply: 3)
  488. siRNA transfection in cardiomyocytes - siRNA transfection in neonatal cultured cardiomyocytes (reply: 1)
  489. Do you have to linearize your vector before transfection? - (reply: 4)
  490. stable transfection of two plasmids? - (reply: 4)
  491. CHO-K1 Transfection - (reply: 1)
  492. a problem of pCMV-Fluc plasmid transfection. - (reply: 1)
  493. NIH 3T3 transfection - (reply: 4)
  494. Trouble with IEC-6 cell transfection - (reply: 3)
  495. Transfection Protocol for HEK293 using GenCarrier-1? - HELP (reply: 9)
  496. trouble with PC12 transfection - (reply: 9)
  497. Help!... Stable transfection.. - A293T with puromycin gene.. (reply: 3)
  498. transfection of HiFive Cells? - Baculovirus Expression (reply: 1)
  499. G418 Stable Transfection - (reply: 4)
  500. Can transfect reagent be frozen? - (reply: 4)
  501. primary culture transfection problems - any transfection methods on primary granulosa cell (reply: 1)
  502. siRNA transfection of non-adherent lymphoma cells - (reply: 2)
  503. hippocampal neuron transfection - (reply: 4)
  504. Transfection, GFP, FACS and UV Microscope - great inconsistency b/t FACS and UV microscope (reply: 3)
  505. SV40-less Luciferase promoter plasmid? - (reply: 2)
  506. Stable transfection of S2R+ and Clone8 cells - (reply: 1)
  507. How to do transfection on 293 cell - (reply: 4)
  508. performing comparison of different transfection agents - (reply: 4)
  509. Need help with transfection before get crazy - Primary human lymphocytes (reply: 2)
  510. Who killed my cells??? - Toxicity during transfection (reply: 3)
  511. plasmid transfection and virus infection simutaneously - (reply: 2)
  512. Transfection reagent for HT-29 and HCT-116 cells - (reply: 4)
  513. MCF-10A transfection question - what to do with slow growing cells. . . (reply: 1)
  514. Transfect/Infect hematopoietic cells - What vector systems to use? (reply: 1)
  515. AtT20 low transfection efficiency - (reply: 4)
  516. plating efficiency for transfection - (reply: 1)
  517. transfection of differentiated Caco-2 cells - (reply: 1)
  518. Co transfection with MCF-7 - help? (reply: 3)
  519. Transfection of keratinocytes with siRNA - (reply: 2)
  520. stable transfection - (reply: 2)
  521. transfection with AS oligos ~ control? - (reply: 7)
  522. siRNA transfection - (reply: 1)
  523. Questions on transfection - (reply: 3)
  524. Interesting gene expression in a stable transfection - (reply: 2)
  525. cell cycle analysis of transiently transfected cells - (reply: 1)
  526. siRNA transfection of primary lymphocytes - (reply: 1)
  527. Transfection of 293T cells on coverslips - (reply: 1)
  528. transfection of 293T w/ SuperFect - help, need info (reply: 5)
  529. MCF7 & MDA MB231 transfection - (reply: 3)
  530. Help! Transfections using Transfectin! - (reply: 2)
  531. Amont of DNA to use for transient Transfection?? - (reply: 2)
  532. Transfect primary cells - is it possible? too difficult? (reply: 2)
  533. help with transfection selection - (reply: 2)
  534. transfection of lymphocytes - (reply: 2)
  535. transfection problem - (reply: 1)
  536. Linearized or unlinearized plasmid for stable transfection? - (reply: 18)
  537. How to get rid of contamination in stable transfected cells - (reply: 3)
  538. Expression screening of stable transfectants? - (reply: 2)
  539. SiRNA transfection in HEK293T - (reply: 1)
  540. Screening stably transfected cells? so little? - (reply: 4)
  541. Lipof/Opti transfection cells died in DMEM/F12 - Transfection (reply: 4)
  542. transfection efficiency - transfection efficiency (reply: 2)
  543. Transfection of CAPAN-1 - Transfection of CAPAN-1 (reply: 1)
  544. Does siRNA transfection dependents on cell type - (reply: 2)
  545. siRNA transfection into neurons - (reply: 2)
  546. Transfection with MIN6 cells - problems with low counts (reply: 1)
  547. siRNA transfection into 3T3-L1 - (reply: 3)
  548. Question about stable transfection of Drosophila Kc-167 cells - stable transfection (reply: 2)
  549. Side effects due to transfection procedures - protein-level change after electroporation (reply: 2)
  550. Luciferase Assay Help!! - (reply: 4)
  551. Transfection - (reply: 1)
  552. transfection of MCF7 and MDAMB231 - how much? (reply: 3)
  553. why linearize plasmids make transfection stable? - (reply: 6)
  554. seeding of cells for transfection - (reply: 4)
  555. What is a mock transfection? - (reply: 4)
  556. Best time for adding G418? - transfection and selecting (reply: 2)
  557. luciferase concentration? - (reply: 5)
  558. luciferase assay - (reply: 3)
  559. transfection problem---SOS! - why the cells should be split before transfection? (reply: 7)
  560. virus vector for transfection - (reply: 8)
  561. Best transfection method/reagent for HeLa cells? - (reply: 4)
  562. transfection-grade plasmid - plasmid extraction or purification kit for transfection (reply: 4)
  563. Transfection of HeLa cell - any advice? please help! (reply: 9)
  564. Normalising luciferase activity - in stable transfectants... (reply: 1)
  565. transfection of Caco2 cells - (reply: 1)
  566. transfection of single copy of plasmid per cell - (reply: 3)
  567. Help, about transfection efficiency - (reply: 7)
  568. Stable Transfection in Sf9 cells - Stable Transfection in Sf9 cells (reply: 5)
  569. How to handle and transfect suspension cells? - (reply: 7)
  570. stable transfection of mouse embryonic stem cells - (reply: 2)
  571. gene linearization for transfection - stable mammalian tranfection. (reply: 1)
  572. Where I find hTert and Tsv40 genes - Stable mammalian transfection (reply: 2)
  573. Transfection with Effectene - (reply: 2)
  574. How to transfect siRNA into the nucleus for transcriptional silencing? - Which transfection reagent? (reply: 9)
  575. How to transfect siRNA into suspension cell lines? - (reply: 3)
  576. Transfection of CaCo2 Cell lines - (reply: 2)
  577. Antibiotics during siRNA transfection - (reply: 4)
  578. Transfection - Cell death after transfection - (reply: 6)
  579. How to prepare enough plasmid for transfection? - (reply: 3)
  580. stable transfection: need of picking single colonies - (reply: 1)
  581. purify linearized plasmid for transfection - (reply: 1)
  582. Different siRNA transfection agents - Have enyone tested them??? (reply: 44)
  583. about vectors and transfection of RNAi - (reply: 1)
  584. efficiently transfect macrophage - (reply: 2)
  585. Help, macrophage transfection - (reply: 2)
  586. Anybody has ever transfected EL4 cells with siRNA? - help! (reply: 3)
  587. transfection on THP-1 cells - a BIG problem (reply: 3)
  588. Can shRNA be transfected without vector...?? - Planning new experiments (reply: 3)
  589. Stable transfection of S2 cells - (reply: 1)
  590. Luciferase assay - (reply: 2)
  591. co transfection for preparing stable cell - (reply: 1)
  592. H295R Cell transfections - (reply: 2)
  593. non-viral transfection of MSCs - non-viral transfection of MSCs (reply: 6)
  594. question about stable transfection - (reply: 1)
  595. siRNA transfection - siRNA transfection (reply: 2)
  596. transfection of thp1 cells ?? - (reply: 3)
  597. CaPO4 transfection prevents trypsin activity - Trying to split after transfecting (reply: 1)
  598. FYI-cell transfection - FYI-cell transfection (reply: 2)
  599. Transfection using PEI - Transfection of 16HBE and CFBE cells (reply: 4)
  600. MC57G cells and transfection - (reply: 1)
  601. transfection efficiency - mammalian cell transfections (reply: 1)
  602. stable transfection with siRNA expression vector - (reply: 6)
  603. HOW TO detect target gene - after the transfection (reply: 5)
  604. help - stable transfection clone pick up - (reply: 33)
  605. the vector for stable transfection - (reply: 3)
  606. Transfection/Infection Problems - (reply: 1)
  607. cell Transfection and western blot - transfection and western (reply: 2)
  608. 293T Transfection Problems/ Apoptosis measurements - (reply: 2)
  609. Transfection - Changing Media - How often do I need to change media? (reply: 1)
  610. Help! Problem with stable transfection of HCT116 - (reply: 4)
  611. How to keep the shape of transfected Hela cell? - Cell transfection (reply: 1)
  612. lipofectin mediated transfectionof HepG2 cells - (reply: 1)
  613. Transient Transfection - Low Transfection Efficiency (reply: 1)
  614. transfection of suspension line cells - method of how to do it (reply: 1)
  615. stable transfection of epithelial cells - stable transfection in mammary breast cell lines (reply: 8)
  616. GFP transfection using lipofectin - (reply: 1)
  617. transfection of suspension typecell - (reply: 3)
  618. Stable transfection of humanFibroblasts - (reply: 1)
  619. stable transfectants - (reply: 1)
  620. plasmid for stable transfection - (reply: 1)
  621. Stable Transfection of large DNAinto 293 cells - (reply: 1)
  622. Stable Transfection of CEM cells - (reply: 1)
  623. Cotransfection - (reply: 1)
  624. stable transfection - (reply: 1)
  625. cotransfection - (reply: 1)
  626. U937 transfection - (reply: 3)
  627. Transfections - (reply: 1)
  628. Transfections - (reply: 1)
  629. Transfection and Western - (reply: 2)
  630. Stable Transfections - (reply: 1)
  631. Transfection - (reply: 2)
  632. which is better - electroporation vs lipofectin - (reply: 4)
  633. transfection - (reply: 2)
  634. transfection - (reply: 2)
  635. Cotransfection problems - (reply: 1)
  636. Transfection in vivo - (reply: 2)
  637. transfection of suspension line cells - methods of how to do it (reply: 1)
  638. Question aboutbouy primary lymphocyte transfection? - (reply: 1)
  639. transfection of atT20 cells - integrin regulation (reply: 1)