siRNA transfection in cardiomyocytes - siRNA transfection in neonatal cultured cardiomyocytes (Sep/29/2005 )
I want to transfect predesigned siRNA(ambion) in cultured cardiomyocytes. I am aware that transfection efficiency is very low in this cell type. Can anybody suggest which transfection reagent should I use to get maximum efficiency. I am planning to use silencer siRNA transefcion regent from Ambion. is it suitable? do anybody know any tip or trick to get efficient transfection in this cell?
-genugene-
My lab has been using RNAicarrier (Epoch Biolabs) to transfect siRNA on cardiomyocytes with satisfactory and consistent results. In addition to Ambion's, I think you may need to test this reagent too.
-BryanW-