hippocampal neuron transfection - (Aug/30/2005 )

Hello,

As for hippocampal neurons transfection (18d old rat embryos neurons in culture), i currently use Lipofectamine 2000. The transfection rate is usually very low (from 2 to 10 transfected neurons out of 40-50000 seeded cells at the beginning).

I intend to try Fugene6 on these cultures, as it works perfectly well on cell lines such as COS-7 or even HEK-293.

Does someone have any experience of this ?

thank you.

Several labs here at CSHL used gencarrier-2 for transfection of primary cortical neurons, PC12, C6..lines. We found this reagent gave us consistently high efficiency and very low cytotoxicity. Compared to lipo2000, fugene6, effectene, gencarrier-2 is better in all aspects.

Hi
I also used Lipfectamine and didn't get enough transfected Hippocampal neurons. Now I usually use Calcum precipitation and it's rate is better than Lipofectamine but not good enough,,
What is Generecin-2?

Gencarrier-2 is a lipoplex transfection reagent (new on market from epoch biolabs, not known to many people), apparently a mixture of cationic lipid, neutral lipid and others.... My colleague recently ordered one and tried on primary chick retinal neurons with ~30-40% efficiency and very low toxicity. Her excperience is that the younger cells the better the efficiency, and do it in serum-free, PBS-free and antibiotic-free medium.

G encarrier-2 is a lipoplex transfection reagent (new on market from epoch biolabs, not known to many people), apparently a mixture of cationic lipid, neutral lipid and others.... My colleague recently ordered one and tried on primary chick retinal neurons with ~30-40% efficiency and very low toxicity. Her excperience is that the younger cells the better the efficiency, and do it in serum-free, PBS-free and antibiotic-free medium.