Calcium Phosphate transfection of HEK293 on poly-lysine plates - (Mar/20/2008 )
Is that possible that the poly-lysine coating inhibits the transfection process?
-Franz K.-
Are cells in good shape? Normally I would not think so, but it is not too hard to prove with a side-by-side experiment.
-genehunter-1-
QUOTE (genehunter-1 @ Mar 20 2008, 03:31 PM)
Are cells in good shape? Normally I would not think so, but it is not too hard to prove with a side-by-side experiment.
It looks pretty fine. Just as normal adherant HEK293 cells on TC plates without coating.
-Franz K.-
Are they an older passage?
-labrat612-