do you coat plates for HEK transfection? - (Jan/11/2007 )
some poeple here in the lab say its necessary to coat with collagen, others say it's ok not to. 
what do you do?
-Kathy-
QUOTE (Kathy @ Jan 12 2007, 11:55 AM)
some poeple here in the lab say its necessary to coat with collagen, others say it's ok not to. what do you do?
what do you do?hi kathy
when i am doing transfection of 293 cells for further lysis exp. i dont coat the plates with collagen but when i want to do transfection for further immunostaining i do coat the plates with collagen.
-spanishflower-
QUOTE (spanishflower @ Jan 11 2007, 09:18 PM)
QUOTE (Kathy @ Jan 12 2007, 11:55 AM)
some poeple here in the lab say its necessary to coat with collagen, others say it's ok not to. what do you do?
what do you do?hi kathy
when i am doing transfection of 293 cells for further lysis exp. i dont coat the plates with collagen but when i want to do transfection for further immunostaining i do coat the plates with collagen.
thank you! I just want to detect if there is a fluorescence or not. so i guess it's ok not to coat.
-Kathy-
when we plate HEK cells for immunofluorescence staining, we donot coat plates.
-scolix-