TRANSFECTION IN HEPG2 - (Nov/01/2007 )

HI Guys,

I am working with gene regulation in fish ovary, I am checking the effect of a particular tarnscription factor on my gene of interest. to chk this I am planing to transfect them into mammal cell line , We have HEPG 2 cell line available in my lab .

The question is : is it ok if I transfect my genes in cell lines which are non fish in origin ? if yes is it ok to use HEPG2 to show the gene regulation which is originally happening in fish ovary? is it a constraint to use ovarian cell lines for ovarian work? is it publishable and acceptable if i use HEPG2 .

please guys reply, if anyone know the answer.
thanks in advance.

regards
Vani

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If the transcription factor has something to do with egg development or regulated by sex dependent/specific mechanism, then I would say it is a bad model to work with.

If you want to check if it functions in a ubiquitous fashion, then yes. HepG2 is not very transfectable, but you can get 30% rate with Lipofectamine 2000.

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Thanks gene hunter,

alright ,can you please also suggest some lines which can be used in this case and which have better transfection rates with lipofectamin 2000.

regards
vani

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293, Cos-7 or Cos-1.

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If you wish to study a particular transcription factor, then use any cell line and it should fine. If its a fish gene and you are using another cell line, I am not sure how comparable they can be. better ask your PI about the relevance of using the HEPG2 cells.

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Thanks a lot for your suggestions.
I inquired around, I got one ovarian cell line CAOV ,is it a good and easly transfected cell line compared to hepg2.

regards
Vani

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