Dual Luciferase Assay -- Problems with reproducibility - (Nov/04/2006 )

I'm using promega's Dual luciferase assay system in the pgl3-basic backbone to study the stress-mediated
induction of a particular promoter in mammalian cells. I have problems with getting repeatable data
using this system. Does anybody else have experience and tips with this?

Hi, I do a lot of dual luciferase. Usually when you dont get reproducible results is because you are not getting a good transfection efficiency, or cells are dying by all the DNA being transfected. Now, can you tell me how your numbers of the constitutive vector (control) are? are they higher or lower than the experimental, do they are in the millions, or hondred thousands.