question about stable transfection - (Sep/26/2004 )

I am doing stable transfection using G418 as the selection reagent. How often should I change the medium? I am changing it once every two days.

Hi,
it depends on your cell growth also, but G418 is stable for about 4 days, so if your cells don't become overconfluent or start looking sick and like they need a "feed" once in 4 days should be ok