medium must be changed after Calcium transfection? - (May/10/2006 )
Hi, guys,
I need to do calcium phosphate transfection on 293T cells. My questions is: is it a must to change the medium (~20 hrs) after transfection?
Bigworm
-bigworm-
I have left the medium on for more than 24 hrs. but then it depends what will you do with the transfected cells. If u r going to produce virus, then u want to change the medium.
-scolix-
QUOTE (scolix @ May 10 2006, 04:44 PM)
I have left the medium on for more than 24 hrs. but then it depends what will you do with the transfected cells. If u r going to produce virus, then u want to change the medium.
I just want to express GABA A receptor (a membrane receptor) and harvest them 60 hrs after transfection.
-bigworm-
I guess it might not make a difference. But the medium might turn yellowish by 60 hrs and the cells might not be healthy. So u could change the media after 20 hrs and then wait for 40 hrs and collect the cells.
-scolix-
hi
pH is case sensitive but maybe also the salot concentration may affect the cells.
-fred_33-