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Buffer Related Discussions
  1. RIPA buffer contents - comparison of 2 buffers (reply: 2)
  2. Dilution Buffer for Elisa - Dilution buffer for titre check of polyclonal serum or for monoclonal (reply: 2)
  3. DNA PAGE - suitability of PCR buffer (reply: 1)
  4. NaPi buffer - NaPi buffer recipie (reply: 1)
  5. Sodium phosphate buffer - (reply: 1)
  6. Ligation buffer - DTT in ligation buffer (reply: 1)
  7. pI and Transfer Buffer - (reply: 1)
  8. Glycine in blocking solution - (reply: 3)
  9. Protein Quantification: Laemmeli Buffer and nanodrop - Laemmeli Buffer and nanodrop (reply: 1)
  10. 1st strand buffer for cDNA synthesis - (reply: 1)
  11. Homogenizing buffer for mouse liver tissue - (reply: 4)
  12. Concentration of EDTA in lysis buffer - (reply: 1)
  13. Processing an thawed aqueous solution without mixing - (reply: 2)
  14. how to prepare phosphate buffer - 1M phospahate buffer (reply: 1)
  15. stripping buffer! - didn't strip after 30min (reply: 3)
  16. Tris Hcl running gel buffer problems - (reply: 6)
  17. RNA sample precipitates when I add the 10X Buffer of the Turbo Dnase (AMBION) - RNA precipitation (reply: 1)
  18. RNA samples precipitates when I add 10X Buffer for Turbo Dnase treatment - RNA precipitation (reply: 2)
  19. protein concentration of samples in Laemmli buffer - (reply: 2)
  20. How to prepare buffer solutions? - I need some help here! (reply: 3)
  21. Laemmli buffer recipe - (reply: 3)
  22. separation of loading buffer - (reply: 1)
  23. Lyticase stock buffer - (reply: 2)
  24. recipe for LDS based sample buffer - (reply: 1)
  25. 1% boiling buffer for protein extractions - (reply: 3)
  26. Volatile Buffers for pH 3 - 10 - Generating a list of volatile buffers for mass spectrometry (reply: 1)
  27. 0.2 M sodium acetate buffer at pH 3.0 - (reply: 2)
  28. EDTA and EGTA into solution - EDTA and EGTA into solution (reply: 5)
  29. How to prepare buffers - (reply: 3)
  30. cell lysis buffer to use directly on the dish - (reply: 1)
  31. 10X Maleic Acid Buffer - (reply: 1)
  32. Proteins recover from different buffer - How to obtain again proteins from ST buffer? (reply: 2)
  33. Autoclaving nuclei isolation buffer - (reply: 8)
  34. SDS buffers - (reply: 1)
  35. SDS buffers - (reply: 1)
  36. SDS buffer - (reply: 1)
  37. Does anyone know how to prepare ammonium molybdate solution? - (reply: 1)
  38. When to use PMSF in a cell lysis buffer? - (reply: 9)
  39. RNA electrophoresis - different concentration of ethidium bromide between gel and buffer (reply: 2)
  40. Why 150 mM KCL in homogenization buffer - (reply: 7)
  41. TBE gel, in TAE buffer. - (reply: 2)
  42. 8M urea buffer for protein extraction - (reply: 6)
  43. What would happen if we don't use protease inhibitors? - for cell lysis by RIPA, NP40 or any other lysis buffer (reply: 8)
  44. Buffer storage question - (reply: 2)
  45. How to calculate Salt concentration in mM scale for any given solution? - (reply: 1)
  46. Dissolve siRNA in non-RNase free buffer - (reply: 1)
  47. Decontaminate RNases from Solution - (reply: 2)
  48. Powdered acrylamide versus solution acrylamide - (reply: 13)
  49. how to make up 85% ammonium sulphate solution - (reply: 6)
  50. SDS buffer - (reply: 2)
  51. SDS Page sample buffer - (reply: 5)
  52. Cell Lysis buffer....do I have to add sodium phosphate to the buffer? - lysis buffer for DNA extractions (reply: 4)
  53. Immunofluorescence - the role of buffers with and without Calcium and magnesium (reply: 4)
  54. Wash solution for Fura-2 AM - (reply: 2)
  55. buffer preparation calculations - (reply: 3)
  56. pH lysis buffer - (reply: 1)
  57. protein isocratic HPLC.. preparatin of tris buffer + n-propanol mix - getting variations in Retention time.. (reply: 1)
  58. Filtration of Acrylamide Solution - (reply: 1)
  59. Ratio between cells and lysis buffer - (reply: 1)
  60. taq Gold and rox buffer - (reply: 2)
  61. Another Dilution Problem... - Molar solutions... (reply: 18)
  62. Cell count in solution - (reply: 2)
  63. Which BSA do I order? - Enzymatic Assay Buffer (reply: 3)
  64. Dilution Problem! - Making up molar solutions.... (reply: 8)
  65. Anyone knows Components of the lysis buffer of Qiagen DNA Isolation Kit - (reply: 1)
  66. IP buffer RIPA - Preserve buffer (reply: 3)
  67. About preparing solution for protein biochemistry - (reply: 2)
  68. Storage of solutions - (reply: 5)
  69. Sample buffer and protein assay - (reply: 1)
  70. Stripping buffer - (reply: 5)
  71. Can we re-use antibody solutions just like Western Blot? - to save antibody (reply: 3)
  72. Multigene cloning - Looking for a cheap solution - (reply: 4)
  73. Na Acetate Buffer calculation - (reply: 1)
  74. transfer buffer - (reply: 1)
  75. Transfer buffer - (reply: 5)
  76. How to sterilize Ovalbumin solution? - (reply: 3)
  77. Digest Buffer Precipitation - Na2HPO4, L-Cysteine, EDTA (reply: 4)
  78. Why is stock solution needed to be sterilized? - Calcium chloride, and Tween 80 ok after autoclave? (reply: 10)
  79. buffers - (reply: 1)
  80. About Hank's balanced salt solution - (reply: 3)
  81. Buffers - (reply: 5)
  82. buffer preparation - (reply: 3)
  83. Blocking buffer recipe - (reply: 5)
  84. Washing buffer and sauton medium - Survival assay in poor condition (reply: 3)
  85. reaction buffer for ABTS - any protocol? (reply: 1)
  86. asking for kanamycin stock solution... - (reply: 4)
  87. protein band is shifted after boiling sample with sample buffer - (reply: 7)
  88. protein band is shifted after boiling sample with sample buffer - (reply: 4)
  89. MAKING A COLLAGEN SOLUTION - (reply: 1)
  90. Sample Buffer - (reply: 1)
  91. buffer preparations - reason (reply: 5)
  92. Dialysis Buffer for Recombinant Protein - (reply: 5)
  93. buffers effect on size exclusion chromatography.. - amm bicarb, phospate with or without salt/ethanol. (reply: 2)
  94. FACS sorting into what solution for RNA isolation? - (reply: 2)
  95. Anode/Cathode buffer in Tris-Tricine SDS-PAGE - purpose of seperate buffers in anode and cathode (reply: 2)
  96. PBS and STE Buffer - (reply: 2)
  97. alternative coating buffers for ELISA - anything else i can use aside from PBS and carbonate/bicarbonate? (reply: 7)
  98. phosphate buffer - (reply: 2)
  99. Lysis buffer - (reply: 1)
  100. RNA gel loading buffer? - (reply: 5)
  101. PBS buffer with or without KCl? - (reply: 2)
  102. Tris or Hepes? - buffer choice (reply: 1)
  103. Cloudy destaining solution...why! - (reply: 5)
  104. how to prepare 0.1 M sodium acetate buffer, pH 5 - i'm bad with my chemistry! (reply: 2)
  105. Can phenol be mixed with QG buffer from Qiagen Kit - (reply: 7)
  106. DMSO in western lysis buffer - (reply: 1)
  107. EROD Assay procedures and solutions experts please help - (reply: 3)
  108. treatment buffer concentration - (reply: 5)
  109. Overshot pH of Competent cell buffer (RbCl) :( - (reply: 3)
  110. digitonin lysis buffer - (reply: 1)
  111. Cloudy Alkaline Phosphatase Buffer! - my AP buffer gets cloudy after autoclave (reply: 6)
  112. Sodium phosphate buffer preparation - pH adjustment (reply: 5)
  113. Lysis buffer for luciferase assay - (reply: 1)
  114. 1N NaOH solution can be autoclaved? - (reply: 13)
  115. Na Acetate buffer recipe - Na Acetate buffer (reply: 2)
  116. Need your suggestion about hybridization solution - The chemical reagants that contained in hybridization solution (reply: 4)
  117. Lysis Buffer for Elisa - (reply: 1)
  118. lipase activity assay - p-nitrophenyl myristate - Turbidity of my substrate solution (reply: 1)
  119. Free trial kit for DNA, RNA, and protein extraction - AquaRNA - an aqueous DNA, RNA, protein extraction solution (reply: 4)
  120. Stripping Solution: Recycle Anyone? - (reply: 6)
  121. How to clean the RNA buffer Tank (to get rid of RNase)? - (reply: 4)
  122. Can gel filtration effect buffer composition? - (reply: 4)
  123. cell lysis buffer - (reply: 4)
  124. Recombinant protein solution turns yellow when concentrated - (reply: 3)
  125. does anyone use pre-chilled SDS buffer? - (reply: 4)
  126. buffer preparation software - beta testers wanted - (reply: 5)
  127. How to choose the right buffer for my western blotting? - (reply: 2)
  128. PBS buffer for cell isolation sing dynabeads - (reply: 2)
  129. protein extraction - cytosolic lysis buffer (reply: 8)
  130. Why hepes buffer turned yellow - (reply: 4)
  131. Recipe for Impron II buffer (Promega) - We have excess enzyme and not enough buffer! (can't belive it myself) (reply: 2)
  132. Molarity of multicomponent buffer solutions - (reply: 3)
  133. Yellow Laemmli Sample Buffer - (reply: 15)
  134. selection of buffer for dialysis? - (reply: 1)
  135. Gummy mess after heating SDS-PAGE sample loading buffer - (reply: 2)
  136. How do I make a blocking buffer solution? - (reply: 1)
  137. Need help to sterilize a solution... - (reply: 6)
  138. Buffers for molecular work - Didn't know where to post (reply: 6)
  139. solution for dilute DNA - Dilution (reply: 1)
  140. TE buffer exposed in the air overnight...is it all right for use? - TO DISSOLVE DNA (reply: 7)
  141. Expiration date for Potassium phosphate buffer? - (reply: 1)
  142. Lysis buffer used to get cell lysates need to be sterilised? - (reply: 8)
  143. Reconstituting lyophilized oligonucelotides - How to make a stock solution with specific molarity, given with a cert (reply: 1)
  144. What is the function of acetate solution in DNA extraction for PCR used - (reply: 6)
  145. RIPA Buffer and DNA - (reply: 3)
  146. HEPES Kinase Assay Buffer - (reply: 2)
  147. Coating buffer for ELISA - Buffer composition needed...Help (reply: 2)
  148. WB reverse image solution?! - white band on darck background (reply: 2)
  149. FACS 5%FBS vs 1%BSA vs FACS Buffer - (reply: 6)
  150. Buffer for cation exchange chromatography - acetic acid or sodium acetate? - (reply: 1)
  151. Preparation of lysis buffer for enzyme extraction - (reply: 5)
  152. TRIS and PBS buffer with ERK (enterokinase) - ERK (enterokinase) (reply: 1)
  153. Cell lysis buffer for real time RT-PCR - Request for cell lysis buffer recipe or relevant scientific paper (reply: 1)
  154. How to quantify protein which has sds buffer already - (reply: 2)
  155. pmsf not dissolving in buffer - (reply: 3)
  156. How would I prepare a single phosphate buffered saline solution from three stock - (reply: 4)
  157. thrombin cleavage buffer - (reply: 1)
  158. Effects of a basic solution on photosynthesis - (reply: 3)
  159. do nanodrop blanking solutions affect readings? - (reply: 2)
  160. protein G, binding buffer pH? - (reply: 3)
  161. PBS buffer - (reply: 2)
  162. Is there ay staining solution to stain colony on agarose gel - (reply: 2)
  163. protein determination in loading buffer - (reply: 1)
  164. ChIP cell lysis buffer - what lysis buffer do you use for Chromatin IPs (reply: 6)
  165. Buffer Exchange & Protein Purification - (reply: 1)
  166. His-tag protein purification- buffers to use - What buffers to choose for His-tag chromatography (reply: 2)
  167. Na-K phosphate buffer recipe? - (reply: 2)
  168. storage buffer for purified protein - (reply: 3)
  169. RIPA as Extraction Buffer in ELISA - (reply: 3)
  170. how to convert molar solutions - (reply: 4)
  171. Making 0.2% acetic acid solution - (reply: 2)
  172. Suitable lysis buffer to extract transmembrane protein? (eg. tyrosinase) - (reply: 11)
  173. Stock solutions? - Does anyone use them?? (reply: 3)
  174. Enzymes in 10 x buffer - (reply: 2)
  175. what's the function of glycerol in co-ip buffer - (reply: 2)
  176. Why HEPES and folic acid in my lysis buffer? - (reply: 4)
  177. On trypsin neutralizing solution - (reply: 2)
  178. Do you add Triton-X in your lysis buffer? - (reply: 7)
  179. recover peptide in solution - question of how to recover peptide about 1500 Da from solution (reply: 1)
  180. pipetting sticky solutions - (reply: 15)
  181. Do you re-use SDS-PAGE running buffer? - (reply: 10)
  182. Protein crashing out of solution - (reply: 4)
  183. Homebrew Hybridization buffers - (reply: 2)
  184. Hybridization Buffer Won't DISSOLVE - (reply: 1)
  185. buffer for anion exchange chromatography of basic proteins - (reply: 1)
  186. how to define an appropriate protein reaction buffer? - (reply: 1)
  187. Sodium phosphate buffer - (reply: 5)
  188. About Tris-Cl buffer - (reply: 4)
  189. Two-color blocking buffer? BSA vs FBS - (reply: 1)
  190. Cloudy mowiol solution - (reply: 1)
  191. Solutions for manual RNA purification - (reply: 2)
  192. Biotin EMSA Blocking buffer - Search for a suitable blocking buffer (reply: 1)
  193. Troubleshoot SB buffer electrophoresis problem - (reply: 6)
  194. commercially available neutralized N-acetyl-L-cysteine solution? - (reply: 1)
  195. buffer preparation - (reply: 5)
  196. how to adjust the PH value of aminocaproic acid solution - (reply: 1)
  197. composition of Qiagen RDD buffer - (reply: 1)
  198. Do antibody solutions settle? - (reply: 2)
  199. Does 20x TTE buffer REALLY expire? - polyacryamide gels; buffers (reply: 1)
  200. when concentrating my protein sample my solution turns a yellow colour ? why? - (reply: 2)
  201. How to prepare thimerosal solution - (reply: 1)
  202. Organelle separation - buffer, homogenization, centrifugation force? - (reply: 1)
  203. SDS-PAGE Loading Buffer with Ficoll? - (reply: 6)
  204. buffer exchange problem - (reply: 3)
  205. Alternatives to Bradford - Bradford disturbed by Lysis Buffer (reply: 3)
  206. Lysis Buffer Preparations - (reply: 3)
  207. 2x SDS-Loading Lysis buffer: use 2x or 1x? - (reply: 4)
  208. purpose of sucrose in buffer - organelle separation - (reply: 2)
  209. washing buffer retained in Ni-NTA column after centrifugation - (reply: 5)
  210. Temperature coefficient : caps buffer - (reply: 1)
  211. Buffer preparation - Problem with EDTA (reply: 3)
  212. Solution Making, Tris, and adjusting pH - before/after? - (reply: 6)
  213. lysis buffer for coip with a membrane protein - (reply: 2)
  214. Tris Tricine Gel Running Buffer - (reply: 1)
  215. Does this buffer suitable for DNA extraction? - (reply: 4)
  216. persistant bacterial contamination, causes and solutions - (reply: 8)
  217. androgen solution for cell treatment - (reply: 7)
  218. How to make EGTA solution? - (reply: 2)
  219. Why the heck my PBS solution is claudy? - I just made PBS adjusted to pH 7.5 (reply: 3)
  220. please give me some suggestions on preparating Yeast cells with PI stainning fo - buffer ,PI solution (reply: 4)
  221. DNA pellet won't go into solution - (reply: 4)
  222. What is buffer AVE ? - (reply: 3)
  223. BUFFER CALCULATION - (reply: 1)
  224. recipe for SB buffer for electrophoresis - (reply: 4)
  225. buffer components - (reply: 2)
  226. affinity purification of antibodies - what is the best buffer to elute (reply: 2)
  227. Help in making up a buffer - (reply: 13)
  228. BCl-2 lysis buffer - BCL-2 lysis buffer recipe (reply: 1)
  229. blocking solution of indirect immunostaining - many days in blocking solution (reply: 3)
  230. buffer for CD - (reply: 1)
  231. how does lysis solution - (reply: 3)
  232. Formaldehyde Fix cells to prevent detergent lysis ? - Testing a protocol to make cells resistant to cell lysis buffer (reply: 2)
  233. I added 2.5 times as much LB to TSS (Transformation and Storage Buffer) - What would happen? (reply: 1)
  234. As, MES - Storage of the solutions: As, MES (reply: 1)
  235. Storage of B glycerophosphate NaF , Na3VO4, Na4P2O7 stock solution for lysis buf - How to store the protease kinase inhibitor soluble stock for lysis buf (reply: 3)
  236. lysis buffer preparation - buffer preparation (reply: 6)
  237. Disinfection with weak solutions of methanol and ethanol? - (reply: 1)
  238. What dialysis buffer should I use for the protein subsequently to be used for ki - What decides the composition of the dialysis buffer? (reply: 1)
  239. calculation problem in preparing the phosphate buffer - (reply: 1)
  240. Low Salt Buffer - (reply: 5)
  241. preparation of Tris HCl buffer - (reply: 2)
  242. Buffer Exchange - (reply: 3)
  243. westerns, protein concentr and RIPA buffer - (reply: 2)
  244. SDS-PAGE sample buffer; SDS solubility - (reply: 2)
  245. Sodium Borate Buffer - (reply: 4)
  246. Buffers for MONO S purification - (reply: 1)
  247. transfer buffer question - anyone ever use TBS as a transfer buffer? (reply: 4)
  248. Standard Transfer Buffer Recipe - Western Blot Transfer Buffer (reply: 2)
  249. lactate buffer - needed composition of lactate buffer (reply: 2)
  250. NADH solution decays on its own? - (reply: 1)
  251. NADH buffer solution itself decays - (reply: 1)
  252. phenol chloroform isoamylalcohol - When do I add the TRIS buffer? (reply: 3)
  253. ELISA/ BSA in capture antibody storage buffer - (reply: 3)
  254. TCA precipitation, what is the best solution to dissolve followed by Mass spectr - (reply: 1)
  255. T4 DNA ligase Buffer - (reply: 3)
  256. Preparing Gel Loading Buffer - (reply: 6)
  257. Solution for a Genome Problem - Calculation of Genome Transformation Probability (reply: 1)
  258. Matrigel invasion assays. Need a fixing solution - (reply: 3)
  259. extraction buffer - (reply: 1)
  260. NP40 lysis buffer - (reply: 3)
  261. primer dissolved in wrong buffer (1M Tris HCl) - what to do now?? (reply: 7)
  262. LOADING BUFFER and UREA ? - (reply: 6)
  263. Removal of detergent from protein solution - (reply: 3)
  264. church buffer - (reply: 1)
  265. APS Solution Stability - Storage at -20°C vs. fresh solution preparations (reply: 11)
  266. problems with phosphate buffer in hydroxyl apatite chromatography - (reply: 1)
  267. denhardt's solution - (reply: 3)
  268. Primer Dilution. ...water or buffer? - (reply: 15)
  269. how to prepare Abeta for cell culture - how to the solution sterile (reply: 1)
  270. Making 0.5mM stock solution of BME - (reply: 3)
  271. pH of western blotting buffers - pH of western blotting buffers (reply: 3)
  272. Buffer QBT - (reply: 2)
  273. Western Blot Incubations - what solution is best for overnight incubations? (reply: 3)
  274. Protein lysis buffer for mass spec - recipe (reply: 15)
  275. Ultrafiltration of Enzyme Solution? - (reply: 4)
  276. Storage stability of hormone solutions - How would you prepare your working solution if your boss thinks that n (reply: 3)
  277. recipe of 0.1M Glycine-NaOH buffer - (reply: 2)
  278. 0,050 M PHOSPHATE BUFFER - (reply: 9)
  279. BORATE BUFFER - (reply: 1)
  280. salt conc in lysis/homogenization buffer? - effect of different salt (NaCl) conc on cell lysis? (reply: 3)
  281. electrophoresis buffer become very hot - (reply: 11)
  282. Making miniprep solutions for spin columns - Now that we can reuse the columns... (reply: 21)
  283. Solution Preparation - (reply: 5)
  284. Buffer Calculator - Good little buffer calculator (reply: 11)
  285. Stringent wash buffer for Immunoprecipitation - What decides stringency of wash buffer when perform IP? (reply: 2)
  286. LDS vs. Laemmli sample buffer - (reply: 1)
  287. SDS Precipitation with 1% Lysis Buffer after 10' on ice - (reply: 1)
  288. Best lysis buffer for mammalian cells to do affinity chromatography? - (reply: 3)
  289. TEMS Buffer preparation - (reply: 1)
  290. What buffer should I incubate HRP secondary? - (reply: 3)
  291. How do you mix solutions in cuvettes - (reply: 5)
  292. Contamination of antibiotic solutions - (reply: 5)
  293. Running Buffer composition in SDS Page - (reply: 7)
  294. Digestion in sub-optimal buffer - (reply: 5)
  295. binding buffer in GST-pulldown - (reply: 1)
  296. cell dead after changing the medium to PBS buffer - (reply: 3)
  297. Electrophoresis buffer prep questions - (reply: 9)
  298. column chromatography - how to make the buffer input rate constant? (reply: 4)
  299. TE Buffer (what did I do wrong?) - (reply: 5)
  300. Solutions, solutions, solutions! - Conversion from %w/w to molar solution (reply: 10)
  301. krebs-ringer buffer preparation - (reply: 1)
  302. did NEBiolab change BamH1's buffer to buffer 3? - (reply: 2)
  303. ELISA - new kit without coating buffer - (reply: 3)
  304. why my freshly prepared 30% Acrylamide solution has precipitate? - (reply: 10)
  305. Difference bwtween BME and DTT in sample buffer for WB after IP - (reply: 10)
  306. RIPA buffer - orthovanadate - (reply: 3)
  307. PCR buffer and PCR efficiency - buffer pH and salt (reply: 2)
  308. dilution buffer for ap labeled 2nd antibody - (reply: 1)
  309. Milky solution after adding 100% ethanol - RNA extraction (reply: 6)
  310. question about plasmid transfection - (water solution or buffer solution?) (reply: 3)
  311. Mix for sequencing - Big Dye buffer necessary or not? (reply: 3)
  312. stable buffer at high pH - (reply: 6)
  313. Lysis buffer ... composition - what's the role of glycerol and/or sucrose? (reply: 2)
  314. BigDye® v1.1 & v3.1 5X Sequencing Buffer Makeup? - (reply: 1)
  315. preciptiate in Fermentas T4 ligase buffer - (reply: 1)
  316. Loading buffer - (reply: 3)
  317. why the buffer contains the EDTA and MnCL2 at the same time? - Help!!! (reply: 6)
  318. Protein pellet won't resuspend in buffer following acetone precipitation - (reply: 4)
  319. SDS-PAGE hi pH/hi Tris loading buffer runs funny - Do proteins generally run funny on Lammelli SDS-PAGE with high pH/high (reply: 1)
  320. Buffer BPTE for DNA electrophoresis - (reply: 3)
  321. regarding the 1X TE buffer - (reply: 5)
  322. Problem with Sodium Borate Buffer - (reply: 8)
  323. DNA isolation from RLT buffer - (reply: 1)
  324. Question about Tris-Hcl and PBS buffer - (reply: 5)
  325. use of Pierce stripping buffer in WB - (reply: 3)
  326. What should be the pH of the leamlli sample buffer? - (reply: 7)
  327. Sucrose in Lysis Buffer? - (reply: 11)
  328. Benedict's Solution question - I need answers to the questions about what the results might be (reply: 1)
  329. glycine or tris buffer - (reply: 5)
  330. Freezing cells in blocking buffer? Urgent! - (reply: 7)
  331. making Ficoll gel loading buffer - (reply: 1)
  332. Storage of buffers - (reply: 3)
  333. digesting DNA with buffer incompatible enzymes - (reply: 4)
  334. Why NaCl is in so many buffers? - (reply: 3)
  335. Hepes buffers turned brown after autoclave - Can I use it? (reply: 5)
  336. why does ligase buffer precipitate? - is it ok just to vortex it?? (reply: 12)
  337. Co-IP..... - What's important for the lysis buffer/washing buffer? (reply: 2)
  338. phosphate buffer.. - na/k/na-k? differences? choice? better? (reply: 2)
  339. buffer and molarity.. choice? - pH 7.0 (reply: 3)
  340. sp-cation exchange column.. - phosphate /tris buffer ph 7.0 (reply: 2)
  341. PH Calibration - Usage of Std Buffers (reply: 1)
  342. sterilization of buffer - (reply: 4)
  343. How to make 1X phosphate buffer, PB? - (reply: 4)
  344. silver stain can even get band with the loading buffer control - (reply: 4)
  345. Western blotting with room temperature buffers - protocol says NOT to use cold buffers (reply: 6)
  346. Trouble with Western blot transfer buffer - (reply: 11)
  347. Dose anyone know IP buffer recipe? - (reply: 6)
  348. Any protocol or methods for estimation of amines in solution - (reply: 1)
  349. effect of TE buffer on cells with cell lysis solution - (reply: 2)
  350. DTT in lämli buffer - (reply: 3)
  351. problem: buffer preparation-molecular biology - (reply: 5)
  352. Flag-tag purifaction elute w/ peptide or SDS loading buffer - (reply: 1)
  353. wash buffer vs lysis buffer PH 7.4 or 8.0 - (reply: 1)
  354. Fe SO4 stock solution - (reply: 7)
  355. Autoclave 1% Triton Solution? - (reply: 6)
  356. Effect of autoclave on solution concentration? - Buffer, sugar, salt (reply: 13)
  357. Purpose of High ionic strength buffers - (reply: 3)
  358. use of Tris-HCL in rehydration buffer - (reply: 1)
  359. Is my RNA dead? - freezing cells after adding RNA lysis buffer (reply: 2)
  360. Problem: High background using RIPA buffer with the bradford assay - (reply: 7)
  361. function of KRB buffer? - (reply: 4)
  362. Transfer Buffer - (reply: 7)
  363. Phosphate Buffer to adjust pH in LB - (reply: 3)
  364. Adenovirus Purification: CsCl solution sould be fresh? - Double CsCl Gradient (reply: 1)
  365. How long can RIPA buffer can be stored at 4C - (reply: 7)
  366. changing dialysis buffer - (reply: 7)
  367. Histidine in buffer, what is the role? - cytosolic and membrane protein isolation (reply: 1)
  368. Does anyone add Etbr to running buffer used to make agarose gels? - (reply: 14)
  369. 1% SDS lysis buffer - (reply: 3)
  370. Filter sterilisation of phytohaemagglutinin solution - (reply: 1)
  371. Resuspended DNA in false buffer! - How to precipitate? (reply: 5)
  372. what these components do in the buffer used in protein purification - (reply: 5)
  373. protein folding- looking for renaturation buffer - (reply: 1)
  374. protein folding- looking for renaturation buffer - (reply: 1)
  375. role of ammonium sulphate in PCR buffer - (reply: 2)
  376. Qiagen gel extraction kit - strange buffer color (reply: 7)
  377. Hygromycin B Stock Solution - (reply: 3)
  378. reconstituted plasmid DNA solution slightly milky - (reply: 3)
  379. Buffer Baffled! - (reply: 3)
  380. An alternative to a PIPES buffer ... - how to dissolve and enzymatically determine lipids (reply: 3)
  381. DNA loading buffer - (reply: 11)
  382. pH buffer - make a pH buffer (reply: 2)
  383. Any database for roles of components in buffers? - (reply: 2)
  384. Buffer to stop grass yellowing from dog urine? - (reply: 6)
  385. Does SDS loading buffer + heat completely denature protein? - (reply: 3)
  386. Maybe dumb question - MgCl2 lysis buffer - (reply: 6)
  387. Electrode Storage solution - Protocol for preparation of Storage solution (reply: 3)
  388. Precipitate in PCR buffer w/BSA & 2-mercaptoethanol - PCR buffers (reply: 1)
  389. Why does changinf buffer effect isolation of RNA? - (reply: 1)
  390. Buffers for Anion Exchange Chromatogrphy - (reply: 8)
  391. Why is EB added into RNA loading buffer? - (reply: 4)
  392. erthrocyte lysis buffer - (reply: 2)
  393. Sorensen's Buffer - (reply: 3)
  394. cell lysis buffer recipe - simple question (reply: 6)
  395. Oxidation of HEPES Buffer Solutions - (reply: 1)
  396. Preparing blocking solution for Western Blotting - what milk power to use? - western blot (reply: 11)
  397. how to remove the foam of BSA solution - (reply: 2)
  398. cell lysis buffer for membrane protein please? - desperately need help.... :S (reply: 8)
  399. what is the function of the sodium azide in the buffer? - western blot (reply: 3)
  400. ABout the solution in Western blot and SDS? - (reply: 5)
  401. DTT in mammilian lysis buffer? - (reply: 12)
  402. temperature stable buffers? - Does snyone know a buffer which has a stable pH at various temperatures? (reply: 3)
  403. pH adjustment for potassium acetate solution - (reply: 1)
  404. E. coli Broth: Sodium phosphate verses Potassium phosphate buffer - (reply: 2)
  405. Making a solution - (reply: 6)
  406. Role of sodium azide in block solution of Western blot - (reply: 4)
  407. how to make this solution? - (reply: 13)
  408. Problem in making SDS-page Sample buffer - (reply: 7)
  409. Acidic buffer for short-time in vitro experiment - (reply: 2)
  410. Protein lysis buffer - Choice of buffer for different proteins (reply: 1)
  411. MSP BUFFER BY Herman et al., - (reply: 2)
  412. Question about stability of bacteria solution - (reply: 2)
  413. Unknown solution - (reply: 3)
  414. Biotinilated proteins. Problems with Hens buffer - (reply: 2)
  415. some protocol - buffer solution (reply: 2)
  416. Washing buffer - cold or warm? - (reply: 6)
  417. Why to use TE buffer for DNA resuspension - (reply: 2)
  418. 3T3 cells and lysis buffer recepie - western blot (reply: 1)
  419. Role of Tris and Glycine in transfer buffer - what are the roles of tris and glycine in transfer buffer? (reply: 4)
  420. Glycerol for enzyme buffer - (reply: 4)
  421. lysozyme solution storage - (reply: 2)
  422. One or two bands with different protein extraction buffer - (reply: 3)
  423. What is the purpose of SDS in microarray hybridization buffers - (reply: 2)
  424. Phosphatase Inhibitors into IP lysis buffer! - (reply: 2)
  425. Concentrate protein from dilute solution - (reply: 2)
  426. what buffer will prevent the precipitation of protein at -20? - (reply: 5)
  427. What is the use of Koch's Lysis Solution? - Question (reply: 8)
  428. how to remove media components from protein solutions - (reply: 4)
  429. Diluent buffer for CIP phosphatase, glycerol conc? - (reply: 1)
  430. Northern Blots - can you reuse the buffers and membrane - Even if its dig labelled (reply: 5)
  431. Tris Buffers vs. pH Electrodes - (reply: 1)
  432. question about lysis buffer - (reply: 3)
  433. SEC for basic protein - column, buffer (reply: 4)
  434. WAHT IS TE BUFFER FOR? - (reply: 9)
  435. Will antibody need any buffer to make link with bead - Which buffer should I use to link bead with antibody (reply: 1)
  436. Silver Staining Protocol for DNA - EDTA solution concentration missing (reply: 1)
  437. buffer for His-tag recombinant protein purification - (reply: 2)
  438. can't stop the precipitate! - adding cacl2 to pk buffer (reply: 6)
  439. RIPA buffer mistake - (reply: 2)
  440. Added DNTP solution to DNA by mistake - (reply: 3)
  441. bacterial pellet and lysis buffer - (reply: 4)
  442. Crystallisation of Phenol saturated solution - (reply: 2)
  443. How to swap buffers? - going from tris-hcl to potassium phosphate (reply: 1)
  444. Question about solution preparation - (reply: 4)
  445. Making buffers - (reply: 5)
  446. concentrating protein from different lysis buffers? - Can you concentrate proteins from differnt buffers and resuspend all in the same (reply: 5)
  447. lysis buffer to extract transmembrane protein - (reply: 3)
  448. Protein quantification: BCA with laemmli buffer? - does it work? (reply: 11)
  449. Difference between salt solution? - (reply: 1)
  450. Problem with DNA extraction from plants - CTAB solution and nanodrop results (reply: 3)
  451. DNA extraction problem - CTAB solution (reply: 2)
  452. interactin between alkaline phosphatase buffer and PNK buffer. - help please (reply: 1)
  453. Whole cell extraction buffer... - does any one have the recipe? (reply: 1)
  454. ELISA wash buffer alternates - (reply: 2)
  455. How to control pH in electrophoresis-----the problem of the buffer - (reply: 2)
  456. SDS sample buffer and serum - why is it solidifying? (reply: 3)
  457. Double digest: efficiency - what does 50% active in given buffer mean? (reply: 1)
  458. Please recommend some solutions for size-exclusion chromatography - (reply: 4)
  459. solution problem - (reply: 5)
  460. Restriction buffer - (reply: 3)
  461. Acrylamide stock for gels - which buffer (reply: 6)
  462. Osmoprotectant for Strep. mutans and buffer - (reply: 1)
  463. SDS and transfer buffer......... - (reply: 1)
  464. about G418 and neomycin - is there any difference? the solution? (reply: 10)
  465. buffer for expression analysis - (reply: 3)
  466. buffer composition - (reply: 11)
  467. Phosphate Buffer - how much buffer to add wastewater (reply: 6)
  468. hepes solution storage - (reply: 4)
  469. PVP: Polyvinylpyrollidone - protein extraction buffer (reply: 3)
  470. strange western - transfer turned PVDF, filter, sponge, and buffer yellow? (reply: 6)
  471. Preparing potassium phosphate buffer(pH 7.4) - (reply: 11)
  472. (Alcian Blue) Staining Solution Preparation - (reply: 1)
  473. mops buffer - (reply: 2)
  474. assay buffers - (reply: 1)
  475. buffer pH unsteady - (reply: 4)
  476. Trouble growing bacteria in LB/amp solution - (reply: 5)
  477. PCR buffers for Taq and Pfu polymerase - (reply: 6)
  478. Making a 80% ethanol/ 20% glacial acetic acid solution - (reply: 3)
  479. Can't make a borate solution. Ideas? - (reply: 4)
  480. problems in concentration protein solution - (reply: 8)
  481. Help me! I need the acidic buffer - (reply: 2)
  482. Guanidine-HCl solution storage - (reply: 1)
  483. loose pellet in prep of electr-competent E.coli - inquire for a solution to solve the problem of loose pellet (reply: 4)
  484. DEPC before or after solution preparation?... - (reply: 4)
  485. function of LiCl in ChIP wash buffer - (reply: 1)
  486. Does this qualify as potassium phosphate buffer? - (reply: 3)
  487. How much dialysis solution? - (reply: 1)
  488. for protein interaction what lysis buffer I should use? - (reply: 1)
  489. can I freeze my samples in loading buffer and use them the next day? - (reply: 2)
  490. How to choose HindIII/EcoRI double digest buffer? - a stupid question, but I really curious about the answer (reply: 6)
  491. TRS Buffer - whats in it?!? - Whats in Dako's TRS? (reply: 1)
  492. Agarose gels and SB Buffer - (reply: 7)
  493. SSC+NaPO4 buffers - (reply: 2)
  494. How to make aurintricarboxylic acid (ATA) solution? - (reply: 1)
  495. Borate buffer recipe - (reply: 16)
  496. question about phosphate buffer? - (reply: 1)
  497. NAD(P)H in solution - handling? - NAD(P)H be frozen and thawed for use in enzymatic assay? (reply: 2)
  498. Co-IP, nuclear proteins - buffers for lysis? - Who else does co-ip on nuclear proteins? (reply: 1)
  499. acetate buffer - (reply: 2)
  500. Phosphate Buffer - (reply: 5)
  501. MSP buffer composition - (reply: 1)
  502. Hank's balanced salt solution, do I need it? - Will PBS do instead? (reply: 3)
  503. preparation of bicine buffer? - (reply: 1)
  504. Water or TE buffer for dissolving DNA - (reply: 5)
  505. Plant DNA celan up - Recipie for clean up solution (reply: 2)
  506. good buffers - (reply: 1)
  507. Difference between adding NaOH to TBE buffer? - (reply: 4)
  508. concentration of SDS loading buffer ... - 1X buffer seems too little. (reply: 8)
  509. calculation for micromolar solution - (reply: 2)
  510. Inoue Methode - How to make the Buffer for the Inou eMethode (reply: 3)
  511. Role of common compounds in different buffers and solutions - compounds are acting in solution as... (reply: 17)
  512. TAE BUFFER - (reply: 2)
  513. science behind commonly used solutions - (reply: 1)
  514. Ambion low conductivity electroporation buffer? - (reply: 1)
  515. Dissolving DNA in TE buffer - is heating necessary? - (reply: 4)
  516. buffer problems - (reply: 1)
  517. how to prepare 0.066 M phosphate buffer? - preparation of buffer (reply: 3)
  518. Potassium phosphate buffer - (reply: 2)
  519. Lysis buffer - (reply: 3)
  520. Buffer for amonium sulphate precipitation - (reply: 1)
  521. how to remove salt from a sugar solution - (reply: 3)
  522. Filtering solutions - (reply: 7)
  523. decontamination of electrophoresis chambers... - what solution should be used? (reply: 2)
  524. Lysis buffer with 1% tritonX - Kindly sugget me its other ingredients (reply: 1)
  525. reducing and nonreducing buffer, SDS-PAGE - (reply: 3)
  526. Gel loading buffer - (reply: 12)
  527. How to accurately quantify plasmid DNA in solution within impurity of RNA? - (reply: 3)
  528. help with buffer 5.1 for RNA extraction - (reply: 6)
  529. Preparing McIlvaine buffer added with potassium chloride - buffer system (reply: 1)
  530. How to concentrate protein that already in SDS buffer and measure concentration? - Help please (reply: 4)
  531. Buffer preparation - Preparation of sodium phospate buffers (reply: 2)
  532. Why do we use methanol in the transfer buffer? - Hope somebody can send me a reply in why do we use methanol in the transfer buff (reply: 3)
  533. Need Help ASAP with maxiprep - Diluted plasmid with buffer ER instead of TE (qiagen kit) (reply: 3)
  534. Role of buffer in antibody purification - (reply: 3)
  535. buffer preparation - (reply: 3)
  536. Strange nanodrop readings after RNA isolation, strange solution - (reply: 13)
  537. water instead of TE buffer to dissolved primers - (reply: 6)
  538. Passive lysis buffer for luciferase assays - (reply: 11)
  539. Making TE buffer - (reply: 6)
  540. to prepare westen blot transfer buffer, instead of methanol can i use ethanol. - (reply: 3)
  541. Condition for sonication of protein solution to get rid of DNA? - How long to sonicate and with what amplitude? (reply: 4)
  542. How to remove LPS from protein solution - (reply: 2)
  543. how to store fixing solution with (para)formaldehyde - (reply: 2)
  544. IP lysis buffer formulation : why adding a non-ionic detergent if a ? - (reply: 4)
  545. Formulation of a buffer solution - (reply: 2)
  546. How to make malonate buffer? - (reply: 1)
  547. How to remove salt from a sugar solution? - Removing KH2PO4 from glucose solution (reply: 7)
  548. DNA, RNA and protein extraction using basic solution - (reply: 4)
  549. Qiagen RPE buffer - (reply: 1)
  550. Why is TE frequently used as buffer for DNA solutions? - (reply: 1)
  551. PBS buffer + HEPES buffer - can be mixed together? - Can I mix two protein in different buffers, same pH? (reply: 2)
  552. PstI restrictase (Invitrogen) - reaction buffer (reply: 1)
  553. Making Tris Buffers of Various pH's by Mixing Tris acid and Tris Base - No HCL needed (reply: 5)
  554. Annealing buffer for DNA oligos - RNA interference (reply: 2)
  555. Collagen solution for coating: do you freeze it? - 4 mg/ml Biochrom AG ready solution (reply: 1)
  556. Ampicillin - Problem in amipicillin solution prep. (reply: 13)
  557. Developing solution at northern blot - (reply: 1)
  558. STRIPPING BUFFER? - how to make good STRIPPING BUFFER? (reply: 3)
  559. why use 2X versus 4X sample buffer? - (reply: 6)
  560. 50mM HEPES-KOH buffer pH7.4 formulation please - (reply: 1)
  561. lysis buffer - (reply: 3)
  562. Lysis Buffer Preparation - (reply: 3)
  563. Can't get PVP into solution - (reply: 1)
  564. FUNCTION OF BUFFER - (reply: 3)
  565. 'Blue loading buffer' - (reply: 6)
  566. Emergent: Toxicity of cell lysis buffer :( - (reply: 2)
  567. Can I add additional sodium ion to PCR reaction buffers? - (reply: 5)
  568. 8-MOP solution - (reply: 2)
  569. how many times do you re-use these two buffers? - (reply: 5)
  570. Role of restriction and TAE Buffer - (reply: 1)
  571. antibody in SDS-containing solution - (reply: 3)
  572. HEPES-KOH buffer - (reply: 5)
  573. digestion with two REs requiring different buffer - (reply: 10)
  574. NMDA buffer toxic? - (reply: 8)
  575. Why freshly prepared alkaline solution II for plasmid extraction? - (reply: 10)
  576. Change Tris for Phosphate buffer cause precipitation - Which buffer can replace Tris? (reply: 3)
  577. Water as an alternative for TE buffer? - (reply: 11)
  578. Stop Solution Doesn't Stop the Reaction - (reply: 3)
  579. His Tag purif buffer-to Tris or not to Tris? - best buffer for pH=7.0 (reply: 1)
  580. buffer preparation for molecular biology - (reply: 9)
  581. Difference between phosphate buffer & potassium phosphate buffer - (reply: 2)
  582. what is the function of Na2EDTA in a buffer solution - (reply: 2)
  583. 50mM Potassium Phosphate buffer, pH 6.0 - (reply: 1)
  584. 0.1M Phospahte Buffer, pH8.5 - Buffer Protocol (reply: 1)
  585. pH balance - HCO3/CO2 buffer in culture media (reply: 1)
  586. loading buffer concentration - (reply: 2)
  587. Proteinase K activation/preparation - water or buffer in proteinase K (reply: 3)
  588. buffer exchange - (reply: 1)
  589. Phosphate buffer - (reply: 6)
  590. Storing of Tris/HCl buffer - (reply: 8)
  591. sterilizing protein solutions + agar - how to sterilize sensitive media (reply: 4)
  592. How to prepare potassium phosphate buffer - (reply: 6)
  593. How To Make 2M Bisulfite Solution? - Bisulfite Sequencing (reply: 6)
  594. orthovanadate in transfer buffer for WB? - immunodetection of phospho ERK1/2 (reply: 6)
  595. TAE Buffer - (reply: 4)
  596. 10% CHAPS solution (10ml) - Recipe wrong or right? (reply: 1)
  597. Stripping Buffer Solution - Stripping Buffer Solution (reply: 3)
  598. phosphate buffer confusion - the true way of preparation pls??!! (reply: 1)
  599. Reuse TAE buffer? - (reply: 6)
  600. Making solutions for protein prep - (reply: 3)
  601. How to prepare 4M ammonium sulphate solution? - Ammonium sulphate precipitation (reply: 5)
  602. 2-mercaptoethanol and TEMED solutions - (reply: 1)
  603. Reducing agent or not in SDS_PAGE sample buffer? - (reply: 2)
  604. shelf life of PMSF/ leupeptin in buffer - (reply: 1)
  605. Reference to Cheeney buffer - (reply: 1)
  606. DNAse and NaF in lysis buffer...a good idea? - Does NaF or Na3VO4 inactivate DNAse I? (reply: 2)
  607. problem with stripping membranes of western blott - do you know some efficient stripping buffer (reply: 3)
  608. What is the role for gelatin in PCR buffer - (reply: 1)
  609. DNase I treatment of RNA dissolved in TE buffer - (reply: 2)
  610. can i autoclave agarose gel with TAE buffer - (reply: 4)
  611. Buffer conditions for Pfu? - (reply: 3)
  612. SDC in buffer? - (reply: 2)
  613. Lysis buffers for brain tissue samples - Which ingredients are superfluous? (reply: 3)
  614. PPT in T4 DNA ligase buffer - (reply: 5)
  615. How to make phosphate buffer? - (reply: 10)
  616. Lysis buffer for membrane protien - (reply: 1)
  617. pH and types of containers for solution storage - (reply: 3)
  618. What is the pH of 2x loading buffer? - (reply: 1)
  619. Buffers for storing and diluting of dNTP - (reply: 3)
  620. TIssue Homogenization Buffer W/o DTT - Missing Reagent (reply: 1)
  621. RIPA lysis buffer (urgent Please) - Na-deoxycholate substitution (reply: 1)
  622. How to prepare primer solution - (reply: 7)
  623. How to increase the strigency of the washing buffer for IP? - (reply: 4)
  624. Problem w/ ECL homemade solution for WB - Final solution works only for a few minutes! (reply: 7)
  625. How to make Britton Robinson buffer? - Britton Robinson buffer receipt (reply: 3)
  626. Which buffers for storing of RNA and DNA? - (reply: 6)
  627. Western Blot: Reducing Buffer Vs Non-erducing buffer - basic Western blot question. (reply: 4)
  628. Is it ok to re-use Western Transfer Buffer - (reply: 4)
  629. protein suspended in diff buffer changes mobility - Will differnt salt concentration in buffer interfe (reply: 2)
  630. Gel extraction and elution buffer - (reply: 4)
  631. separation of nucleus protein - lysis buffer (reply: 1)
  632. Recipe for anti-fade solution?? - (reply: 1)
  633. can i autoclave buffers with imidazole for His-tag purification? - (reply: 2)
  634. His-taged protein is in 8M Urea, how to get rid of urea? - Ni-NTA column purified protein in 8M urea buffer (reply: 3)
  635. In situ Hybridization problem with TCS solution?? - (reply: 1)
  636. Help: western blot lysis buffer - (reply: 2)
  637. Lysis buffers for extraction of proteins expressed in E.coli - (reply: 5)
  638. SDS-PAGE loading buffer - 1X, 2X, 4X loading buffer? (reply: 2)
  639. Dissolving DNA in TE buffer - troubleshooting - (reply: 3)
  640. lysis buffer for western blot - lysis buffer (reply: 3)
  641. a recipe of a lysis buffer for co-IP (phosphorylation) - can anyone recommend one? (reply: 3)
  642. what these chemicals for in lysis buffer? - (reply: 3)
  643. Is TE buffer toxic to cultured cells? - cell culture (reply: 4)
  644. About the maleic acid buffer - Maleic acid buffer can be autoclave? (reply: 1)
  645. extraction buffer - (reply: 1)
  646. Phosphate buffers - protein and NaCl affecting pH of buffer (reply: 4)
  647. which buffer need to autoclave, which can not - (reply: 1)
  648. preparation of solutions that need pH adjustment - (reply: 3)
  649. NP40 concentration in protein lysis buffer - (reply: 1)
  650. Balanced salt solutions - (reply: 2)
  651. smear of loading buffer observed - SDS-PAGE gel (reply: 5)
  652. How do you prepare PCR stock solution to minimize pipetting - (reply: 37)
  653. How to make a tetracycline solution - (reply: 2)
  654. Plasmids in eluting buffer - (reply: 1)
  655. Make a solution of 10,000ppm hypochlorite - (reply: 9)
  656. preparing buffer - (reply: 3)
  657. MMP assay - proteases in lysis buffer (reply: 1)
  658. Azacytidine mother solution - (reply: 5)
  659. dsOligos annealing buffer - (reply: 2)
  660. Buffer for red blood cell lysis for RNA extraction from blood - RNA methodology (reply: 6)
  661. Sample Buffer Recipe (Laemmili) - (reply: 5)
  662. Good buffer needed! - (reply: 7)
  663. Can I use 4M urea buffer in DEAE chromatography? - (reply: 1)
  664. How to prepare solution for drug or hormone treatment?? - Preparing dexamethasone and estradiol solution from powder (reply: 4)
  665. Making TE buffer for RNA disolution - afraid of RNase contamination (reply: 3)
  666. Difference between TE buffer at pH 8.0 and 7.5 - (reply: 2)
  667. Protein precipitated after buffer exchange - (reply: 3)
  668. Reuse TAE buffer and agarose gel? - yes or no... (reply: 22)
  669. Coating Buffer for Sandwich ELISA - please help! (reply: 7)
  670. Alginic acid - How to get it into solution? (reply: 4)
  671. About 5' aza-cytidine - stock solution (reply: 2)
  672. Do enzymes work in different buffers? - (reply: 2)
  673. MES Buffer Receipe - (reply: 2)
  674. hydroproline assay citrate buffer - (reply: 1)
  675. SDS electrod buffer - SDS electrod buffer (reply: 2)
  676. Convert DNase units to weight - 2mg/ml from a 2U/ml solution (reply: 3)
  677. Electroblotting of Proteins on - transfer buffer without Tris and Glycine (reply: 1)
  678. Preparation of Estrogen solution - (reply: 2)
  679. Na2Hpo4 , NaH2po4 or both in Church buffer ?! - (reply: 2)
  680. can RIPA buffer use to prepare nuclear protein? - (reply: 1)
  681. Concentrating proteins from solution - (reply: 2)
  682. How to prepare the balance solution - (reply: 1)
  683. Ammonium Sulfate PCR buffer - Ammonium Sulfate PCR buffer (reply: 1)
  684. Lysis Buffer for MTT - (reply: 1)
  685. Fura 2 loading - Fura 2 /AM solution in DMSO loading in Tcells (reply: 1)
  686. what solution to use for cell lysis - (reply: 5)
  687. Chromatofocuusing - How to make a pH gradiem (3-8) by using simple buffers. (reply: 1)
  688. endotoxin free buffers - (reply: 1)
  689. Hank's solution - (reply: 2)
  690. Buccal swab storage - is saline solution enough? (reply: 1)
  691. electrophoresis buffer - when I should replace running buffer (reply: 1)
  692. cell lysis - How do I decide what solution to use? (reply: 2)
  693. What is the difference between 2x and 4x sample buffer? - SDS Sample buffer (reply: 1)
  694. MES buffer in dialyzation - MES or any alternative for MES? (reply: 1)
  695. solution lysis - (reply: 2)
  696. Why use imidazole as a buffer? - Imidazole Buffer (reply: 2)
  697. How to make PCR  using the gel solution - (reply: 1)