pH lysis buffer - (Sep/15/2008 )
Hello!
Is there any specific reason why lysis buffers usually have a pH = 7.5? Since I load my protein extracts on SDS gels, would it be possible to lyse the cells with Tris pH = 6.8? :-)
Cheers,
Alex
-sephadex-
QUOTE (sephadex @ Sep 15 2008, 08:59 AM)
Hello!
Is there any specific reason why lysis buffers usually have a pH = 7.5? Since I load my protein extracts on SDS gels, would it be possible to lyse the cells with Tris pH = 6.8? :-)
Cheers,
Alex
Is there any specific reason why lysis buffers usually have a pH = 7.5? Since I load my protein extracts on SDS gels, would it be possible to lyse the cells with Tris pH = 6.8? :-)
Cheers,
Alex
it is near intracellular cytosolic pH, and prevents pH-dependent denaturizing during lysis for most proteins...
-The Bearer-