Protein Quantification: Laemmeli Buffer and nanodrop - Laemmeli Buffer and nanodrop (Dec/16/2008 )
Hello,
I am new to using the nanodrop and just want to do a quick protein estimation using the 280 nm program on nanodrop. Does anyone have a protocol ? My samples are in 2x laemmeli buffer with SDS, BME, and bromophenol blue. Will these chemicals hurt the nanodrop or provide grossly inaccurate measurements?
thanks for any help!
-dkw066-
bromphenol blue will interfere with the readings.
without the bpb you could have blanked with the sample buffer mixed with laemmli buffer then read the sample.
-mdfenko-