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Protein Quantification: Laemmeli Buffer and nanodrop - Laemmeli Buffer and nanodrop (Dec/16/2008 )

Hello,
I am new to using the nanodrop and just want to do a quick protein estimation using the 280 nm program on nanodrop. Does anyone have a protocol ? My samples are in 2x laemmeli buffer with SDS, BME, and bromophenol blue. Will these chemicals hurt the nanodrop or provide grossly inaccurate measurements?
thanks for any help!

-dkw066-

bromphenol blue will interfere with the readings.

without the bpb you could have blanked with the sample buffer mixed with laemmli buffer then read the sample.

-mdfenko-