EDTA and EGTA into solution - EDTA and EGTA into solution (Nov/11/2008 )

What is the saturation point? Would it be impossible to make a 600 mM EGTA and 600 mM EDTA solution?

For 100 mL this would involve 22.9 g EGTA and 22.3 g EDTA in 100 ml and adjustment to ph8.

Id like to know if its possible to go that high, because Ive heard getting them into solution can be difficult.

Thanks!

Hey,

I assume that is related to your earlier questions about the calculations for your DNase inactivation.
So I think you can go higher than that. I would def. try a 1M solution. However, you have to add a little bit of NaOH. Otherwise you will not get it into solution.

You might have to heat it a little bit.

We routinely make up 500 mM EDTA, but be careful when adding your NaOH - it sometimes requires a lot depending on how you are adding it (5N, 10N, pellets). If you add too much water to begin with then your concentration will be off.

Thanks! All very helpful