Buffers - (Jul/11/2008 )
Hi
I have made a buffer comprising Tris HCl and CaCl2 for enzymatic reactions, but after 2 wks it has turned blue . I have kept the buffer at room temperature (370C). Can any one explain me why it has turned to light blue colour.
Sallie
I have made a buffer comprising Tris HCl and CaCl2 for enzymatic reactions, but after 2 wks it has turned blue . I have kept the buffer at room temperature (370C). Can any one explain me why it has turned to light blue colour.
Sallie
must be a hot summer if there are 37°C in your lab
your components should not cause this effect; may be impurities or contaminations like Cu2+ from the ingredients or bottle...
Your lab is very warm...37degree!
Sounds like contamination, are you sure it is blue, not just cloudy/scattering light so that makes it look a bit blue?
Hi
Cacl2 and Tris buffers allow easy growth at room temperatures (26-370C). so keep you your Tris and Cacl2 buffers at 40C and whenever you want to use , bring the buffer to room temperature and use it
All the best
Regards
S
I have made a buffer comprising Tris HCl and CaCl2 for enzymatic reactions, but after 2 wks it has turned blue . I have kept the buffer at room temperature (370C). Can any one explain me why it has turned to light blue colour.
Sallie
What Shan said!!!
- Throw the buffer out
- Make a new buffer
- Sterilize it
- STORE IT AT 4 C
and for your own sake... Turn the thermostat in your lab DOWN TO 23 C