DNA, RNA and protein extraction using basic solution - (Nov/29/2005 )
Can someone help explain to me how using a basic solution of 0.2 N NaOH in cell extractions works when trying to extract proteins, DNA, and RNA? I cant find any books that can clearly point out the effts on each macromolecule.
that question is pretty open-ended, but I am guessing that what you are looking for is this:
alkaline solutions will lyse bacterial cell walls, 'blowing the cells open' so to speak...this allows for subsequent extraction of the molecules you listed.
to get the DNA, RNA, proteins out of the cells, you have to crack them open first, and NaOH will do this
does that help?
alkaline solutions will lyse bacterial cell walls, 'blowing the cells open' so to speak...this allows for subsequent extraction of the molecules you listed.
to get the DNA, RNA, proteins out of the cells, you have to crack them open first, and NaOH will do this
does that help?
IM MAINLY ASKING WHAT WOULD BE THE EFFECT OF NaOH ON THE THREE MACROMOLECULES. ARE THEY STABLE IN A BASIC SOLUTION? WOULD THEY DENATURE IN A BASIC SOLUTION? AND IF SO WHY? I HAVE READ EVER BOOK I HAVE AND SEARCHED THE WEB AND I CANT SEEM TO GET A CLEAR ANSWER :-(.
wow, that sounds like a good question for a weekend curled up with a cup of coffee and a biochem or organic chem text
I'm sorry, I could spend many hours digging it out for you, but then my boss would kill me for not getting any work done
good luck with your question; sorry I can't help you further 
In 0.2 N NaOH, DNA will be denatured into single-stranded, and RNA will be degraded into small fragments because RNA is labile in basic solution. I am not sure what will happan to the protein.