How to clean the RNA buffer Tank (to get rid of RNase)? - (May/05/2008 )

Dear all,

I would like to clean my RNA buffer Tank and comb. What should I do? Clean it by detergent and then 0.2M NaOH and DEPC water?

Any opinion?

Many Thanks

siuchi

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3% hydrogen peroxide is probably easier. Then DEPC water.

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Dear phage434,

Thank you for your kind reply. Our hydrogen peroxide was expired, any alternative? Thanks so much.

siuchi

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Hey,

i do it always with 96% EtOH. So far I have had good results.

cheers,

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hi siuchi..

i've been doing rna extraction in my lab n we have been using this method for quite a while.i think it works for us ()...

1: soak the well tank with 0.2% SDS(overnite)
2: rinse with d H2O
3: soak with 100% EtOH (20 minutes)
4: rinse with d H2O
5: soak with DEPC water (1 minute)
6: ready to use

i'm quite new in this forum..so hopefully it helps...

noreen...

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