denhardt's solution - (Jun/04/2007 )

anyone knows how to make denhardt's solution for hybridization after southern blot....without buying a kit?

Hi Solmaniar,

here's the recipe for 100x Denhardt's solution:


10g Ficoll 400
+ 10g Polyvinylpyrrolidone
+ 10g Bovine Serum Albumin
fill up to 500 ml with H2O

Greetings,
Chakchel

by googling i got some recipies. we use powder from a kit of GE healthcare

Thank you very much!!!!