Wash solution for Fura-2 AM - (Sep/17/2008 )
Hi. Can anyone please help me with the protocol for using Fura-2 am? What solution should be used to wash the cells? I have been going through literature and cant seem to find a consistently-used buffer. Some use HBSS, some use d-Hanks, some use a HEPES buffered solution, some seem to make up their own solutions. Does it make a difference which one you use? i'm working with U937 cells. Thanks for any advice! 
-shmelly-
QUOTE (shmelly @ Sep 17 2008, 10:57 AM)
Hi. Can anyone please help me with the protocol for using Fura-2 am? What solution should be used to wash the cells? I have been going through literature and cant seem to find a consistently-used buffer. Some use HBSS, some use d-Hanks, some use a HEPES buffered solution, some seem to make up their own solutions. Does it make a difference which one you use? i'm working with U937 cells. Thanks for any advice!
Hi, I use HBSS to wash my cells after incubation in Fura-2AM. All of the buffers you mentioned are buffered salt solutions, and it shouldn't matter which one you use. I use HBSS to wash my cells in cell culture, so maybe you could use whichever solution you use for that.
Hope this helps!
-Obsidian-
Thanks!
-shmelly-