buffer preparations - reason (Jun/30/2008 )

wat is the reason to say abaout a buffer with 1X, 10X , etc., in staed of 10mM,50mM,etc., of cource these are also we can take.
vijaya

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I am not clear as to what your question is. However, some manufacturers produce buffers in concentrate form for shipping. The concentrates are simply diluted in water and no calculations are required.

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To make life easy! If a buffer is made with a particular reaction in mind, it is an easy way.

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10x buffer is just like concentrated juice/cordial that you need to dilute with water.

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Typically buffers that use the 1X, 10X etc notation are made up of several components, each with a different concentration, so it is easier to call it, for example "10X buffer A" than to call it 50 mM Tris, 25 uM EDTA, 5% glycerol, 1 M glycine each time.


Note buffer is not real, just an example.

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[quote name='vijaya24' date='Jun 30 2008, 07:10 AM' post='141588']
[indent]

wat is the reason to say abaout a buffer with 1X, 10X , etc., in staed of 10mM,50mM,etc., of cource these are also we can take.
vijaya

hi,

a 1X, 10X,...buffer is a concentrated buffer(with all its components) that you have to dilute in water or in the solvent mentionned on the notice
for exemple, if you have a 10X buffer and you are preparing a total volume of 200ml, you need 20ml of the buffer and 180ml of water to complete 200ml

talking about 10mM, 50mM, etc is the molarity or if you want the concentration of one compound in a solution
for example, if you want to prepare 1L of 10mM NaOH solution, you proceed like this:
m
M = -------- ---> m = M X MW X V = 0.01M X (23+6+1) X1L = 0.4g
MW X V
MW is the molecular weight of each atom

so, weigh 0.4g of NaOH and dissolve it in 1L of water and you have a 10mM NaOH solution

hope it's clear!

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