Protocol Online logo
Top : Forum Archives: : Biochemistry

How to calculate Salt concentration in mM scale for any given solution? - (Oct/07/2008 )

Dear all,
A buffer I am using is:
65 mM Tris-Hcl
0.5% SDS
Is the salt concentration 65 mM then?

How about PBS?, which, for a liter, has
8g of NaCl
0.2g of KCl
1.44g of Na2HPO4
0.24g of KH2PO4

should I just simply calculate the molar concentration of each and plus these four together to get the salt concentration of the whole buffer?

thanks rolleyes.gif

-shawnlightr-

QUOTE (shawnlightr @ Oct 7 2008, 02:18 PM)
Dear all,
A buffer I am using is:
65 mM Tris-Hcl
0.5% SDS
Is the salt concentration 65 mM then?

How about PBS?, which, for a liter, has
8g of NaCl
0.2g of KCl
1.44g of Na2HPO4
0.24g of KH2PO4

should I just simply calculate the molar concentration of each and plus these four together to get the salt concentration of the whole buffer?

thanks rolleyes.gif

q1: SDS is also a salt (sodium!); you must transform % in mM and add it to 65 mM
q2: I would also do it the same way....

-The Bearer-