Do enzymes work in different buffers? - (Jan/05/2005 )
Hi!!
Can I do an Klenow-Polymerase and following SAP-treatment in the same buffer as my restiction digest?!
Restriction digest buffer conditions: 20mM Tris (pH 8.5); 20mM MgCl; 1mM DTT; 100mM KCl.
I heard that Klenow-Pol works pretty much in any buffer, is that right?!
Happy science!!
-petraparker-
Different enzyme needs different kind of circumstances.
you can find some information from NEB company.
newdragon
-newdragon-
Klenow and SAP will work in most restriction buffers. When I cut vectors, I routinely perform the digestion in the presence of SAP.
-tfitzwater-