How to make a tetracycline solution - (Mar/25/2005 )
I am making at 5 mg/mL soln of Tetracycline, and am using EtOH per our most recent Molecular Cloning manual...but I am having trouble getting all of the solid dissolved. Should I be using 70 % ETOH? Any thoughts/suggestions would be appreciated!!
-slapolla-
Ive made some Tet sol. a couple of times....be sure to use an analytical balance, and dissolve it in absolute ethanol (100%)....and shake it up like theres no tomorrow...itll take a while but it will go into solution...
Do not autoclave...its ready for use....
Cheers
QUOTE (slapolla @ Mar 25 2005, 10:40 AM)
I am making at 5 mg/mL soln of Tetracycline, and am using EtOH per our most recent Molecular Cloning manual...but I am having trouble getting all of the solid dissolved. Should I be using 70 % ETOH? Any thoughts/suggestions would be appreciated!!
-matiascs-
i agree with matiascs. I do the same
chandima
-chandima-
People,
Err... How do we know when to use water or ethanol? We have so much stocks here that I have yet to prepare any antibiotic solutions.
-dreamchaser_jc-
QUOTE (dreamchaser_jc @ Jan 29 2009, 09:54 AM)
People,
Err... How do we know when to use water or ethanol? We have so much stocks here that I have yet to prepare any antibiotic solutions.
Err... How do we know when to use water or ethanol? We have so much stocks here that I have yet to prepare any antibiotic solutions.
you use that in which it is soluble and stable and will not interfere with your purpose.
-mdfenko-
QUOTE (mdfenko @ Jan 30 2009, 12:53 AM)
QUOTE (dreamchaser_jc @ Jan 29 2009, 09:54 AM)
People,
Err... How do we know when to use water or ethanol? We have so much stocks here that I have yet to prepare any antibiotic solutions.
Err... How do we know when to use water or ethanol? We have so much stocks here that I have yet to prepare any antibiotic solutions.
you use that in which it is soluble and stable and will not interfere with your purpose.
Alright, got it!
-dreamchaser_jc-